| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NADPH oxidase 4, 163-, Kidney oxidase-1, KOX-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase, NOX4, RENOX |
| Entrez GeneID | 50507 |
| WB Predicted band size | 66.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NOX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-153 amino acids from the N-terminal region of human NOX4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及NOX4(N-term)抗体的文献摘要信息(均为虚构示例，供参考格式)：
1. **"NOX4-derived ROS promote renal fibrosis via TGF-β1 signaling"**
- **作者**: Smith A et al. (2020)
- **摘要**: 研究使用N端特异性NOX4抗体(货号AB123)通过免疫组化检测肾脏组织中NOX4表达，发现NOX4通过ROS-TGF-β1轴驱动纤维化。
2. **"N-terminal antibody reveals NOX4 localization in mitochondrial membranes"**
- **作者**: Lee C et al. (2019)
- **摘要**: 利用N-term抗体(兔多克隆)进行亚细胞定位分析，证实NOX4在线粒体内膜的表达，并参与氧化应激诱导的细胞凋亡。
3. **"NOX4 inhibition attenuates tumor angiogenesis in breast cancer models"**
- **作者**: Zhang Y et al. (2021)
- **摘要**: 通过Western blot(NOX4 N-term抗体，Sigma XX456)验证基因敲除效果，证明NOX4通过调节VEGF通路促进肿瘤血管生成。
(注：以上文献为示例模板，实际引用需替换为真实文献，建议通过PubMed或Web of Science搜索关键词“NOX4 antibody N-terminal”获取具体信息。)
The NOX4 (N-term) antibody is a tool designed to detect the N-terminal region of NADPH oxidase 4 (NOX4), a key enzyme in reactive oxygen species (ROS) generation. NOX4. a member of the NADPH oxidase family, is constitutively active and primarily produces hydrogen peroxide (H₂O₂), influencing cellular signaling, differentiation, and stress responses. Unlike other NOX isoforms, NOX4 is unique in its dependence on regulatory subunits (e.g., p22phox) and its localization to intracellular membranes, including mitochondria and endoplasmic reticulum. The N-terminal domain of NOX4 plays a role in protein-protein interactions and subcellular targeting, making it critical for enzymatic activity and functional specificity.
The NOX4 (N-term) antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study NOX4 expression, localization, and regulation in tissues or cells. Its specificity for the N-terminus helps distinguish NOX4 from other NOX isoforms, minimizing cross-reactivity. Researchers employ this antibody to explore NOX4's involvement in pathological processes such as fibrosis, inflammation, cancer progression, and cardiovascular diseases, where dysregulated ROS production contributes to tissue damage or signaling abnormalities. Validation of the antibody typically includes knockout controls or peptide blocking assays to confirm target specificity. Understanding NOX4's role via this antibody has advanced insights into redox biology and potential therapeutic targeting of ROS-related disorders.
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