| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NACHT, LRR and PYD domains-containing protein 12, Monarch-1, PYRIN-containing APAF1-like protein 7, Regulated by nitric oxide, NLRP12, NALP12, PYPAF7, RNO |
| Entrez GeneID | 91662 |
| WB Predicted band size | 120.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NLRP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-212 amino acids from the N-terminal region of human NLRP12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于NLRP12 (N-term)抗体的3篇参考文献，包含文献名称、作者及摘要核心内容：
1. **"NLRP12 suppresses colon inflammation and tumorigenesis through the negative regulation of NF-κB signaling"**
- **作者**: Zaki, M.H., et al.
- **摘要**: 本研究利用NLRP12 (N-term)抗体进行Western blot和免疫共沉淀实验，验证了NLRP12通过抑制NF-κB通路调控结肠炎和结肠癌的发生，抗体特异性通过基因敲除小鼠模型得到确认。
2. **"The NLRP12 inflammasome recognizes Yersinia pestis via dual pathway activation"**
- **作者**: Vladimer, G.I., et al.
- **摘要**: 通过NLRP12 (N-term)抗体进行免疫荧光染色和蛋白质互作分析，揭示了NLRP12在鼠疫杆菌感染中的双重激活机制，抗体特异性通过siRNA敲降实验验证。
3. **"NLRP12 attenuates tumorigenesis in head and neck squamous cell carcinoma by inhibiting inflammasome activation"**
- **作者**: Allen, I.C., et al.
- **摘要**: 使用NLRP12 (N-term)抗体检测头颈鳞癌组织中NLRP12的表达水平，发现其通过抑制炎症小体活化抑制肿瘤进展，抗体验证包括肽段阻断实验和质谱分析。
以上研究均涉及NLRP12 N端抗体的实验应用，涵盖疾病模型中的功能验证及分子机制探索。
The NLRP12 (N-terminal) antibody is a specialized tool for detecting the N-terminal region of the NLRP12 protein, a member of the NOD-like receptor (NLR) family. NLRP12. also known as Monarch-1. is an intracellular pattern recognition receptor involved in innate immunity and inflammatory responses. It contains a pyrin domain (PYD) at its N-terminus, a central NACHT domain responsible for ATPase activity, and a leucine-rich repeat (LRR) domain at the C-terminus. The N-terminal PYD facilitates protein-protein interactions, particularly with adaptor molecules like ASC, to regulate signaling pathways such as NF-κB and inflammasome activation.
NLRP12 functions as a negative regulator of inflammation, modulating immune responses to pathogens and cellular stress. It is implicated in diseases like familial cold autoinflammatory syndrome (FCAS), colitis, and cancer. The NLRP12 (N-term) antibody is commonly used in research to study protein expression, localization, and interaction partners via techniques like Western blotting, immunohistochemistry, and immunoprecipitation. Its specificity for the N-terminal region ensures targeted detection, distinguishing NLRP12 from other NLR family members. Studies using this antibody have advanced understanding of NLRP12’s dual roles in suppressing pro-inflammatory pathways and promoting immune homeostasis, highlighting its potential as a therapeutic target for inflammatory and autoimmune disorders.
×
