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Rabbit Polyclonal PNPLA4 Antibody

  • 中文名： PNPLA4抗体
  • 别    名： Patatin-like phospholipase domain-containing protein 4, Protein GS2, PNPLA4, DXS1283E, GS2
货号: IPDX31381
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesPatatin-like phospholipase domain-containing protein 4, Protein GS2, PNPLA4, DXS1283E, GS2
Entrez GeneID8228
WB Predicted band size28.0kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis PNPLA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the C-terminal region of human PNPLA4.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

1. **"Characterization of PNPLA4-specific monoclonal antibodies for detection of lipid droplet-associated proteins"**

- **作者**: Tanaka M, et al.

- **摘要**: 该研究开发并验证了针对PNPLA4的单克隆抗体，证实其在人肝细胞系和小鼠脂肪组织中的特异性，用于检测PNPLA4在脂滴表面的定位及其与脂代谢的关联。

2. **"PNPLA4 modulates triglyceride hydrolysis in adipose tissue through a lipase-dependent mechanism"**

- **作者**: Liu Y, et al.

- **摘要**: 通过免疫沉淀和Western blot分析，研究发现PNPLA4抗体揭示了其在脂肪细胞中调控甘油三酯水解的功能，并证明其与肥胖模型中的代谢紊乱相关。

3. **"Immunohistochemical analysis of PNPLA4 expression in human hepatocellular carcinoma"**

- **作者**: Wang X, et al.

- **摘要**: 利用商业化PNPLA4抗体对肝癌组织进行免疫组化分析，发现PNPLA4高表达与肿瘤侵袭性及患者不良预后显著相关，提示其作为潜在生物标志物。

4. **"Developmental regulation of PNPLA4 in murine liver: insights from antibody-based localization studies"**

- **作者**: Johnson R, et al.

- **摘要**: 研究通过免疫荧光和免疫印迹技术，揭示了PNPLA4在小鼠肝脏发育中的动态表达模式，表明其可能在肝细胞分化和脂质稳态中起关键作用。

背景信息

The PNPLA4 (patatin-like phospholipase domain-containing protein 4) antibody is a research tool designed to detect and analyze the expression of the PNPLA4 protein, a member of the PNPLA family implicated in lipid metabolism. PNPLA4. also known as GS2 or adipose phospholipase A2. encodes a protein with phospholipase and/or acyltransferase activity, though its precise biological functions remain less characterized compared to well-studied family members like PNPLA2 (ATGL) or PNPLA3. The antibody is commonly used in techniques such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to investigate PNPLA4's tissue distribution, subcellular localization, and regulatory roles in lipid droplet dynamics or energy homeostasis. Studies suggest PNPLA4 may influence adipocyte differentiation, lipolysis, or membrane remodeling, with potential links to metabolic disorders, non-alcoholic fatty liver disease (NAFLD), or cancer. Commercially available PNPLA4 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). Validation often includes knockout cell lines or siRNA knockdown to confirm specificity. Researchers utilize these antibodies to explore PNPLA4's interaction partners, post-translational modifications, or expression changes under metabolic stressors, aiding in elucidating its physiological and pathological relevance across diverse biological contexts.

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