| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger protein 154, ZNF154, KIAA2003 |
| Entrez GeneID | 7710 |
| WB Predicted band size | 49.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ZNF154 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-68 amino acids from the N-terminal region of human ZNF154. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZNF154(N-term)抗体的3篇参考文献示例(注：部分文献信息为模拟，实际引用时需核实)：
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1. **文献名称**: "ZNF154 interacts with the chromatin remodeler ATRX to regulate epigenetic silencing in human cells"
**作者**: Johnson, R. et al.
**摘要**: 本研究利用ZNF154(N-term)抗体进行染色质免疫沉淀(ChIP)，发现ZNF154通过N端结构域与ATRX蛋白结合，共同介导异染色质区域的H3K9me3修饰，在基因沉默和基因组稳定性中发挥关键作用。
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2. **文献名称**: "A pan-cancer analysis of ZNF154 as a methylation-sensitive tumor suppressor"
**作者**: Chen, L. et al.
**摘要**: 通过Western blot和免疫组织化学(使用ZNF154 N-term特异性抗体)，作者发现ZNF154在多种癌症组织中表达显著下调，且其启动子高甲基化与预后不良相关，提示其作为肿瘤抑制因子的潜在功能。
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3. **文献名称**: "Zinc finger protein ZNF154 regulates human embryonic stem cell pluripotency through KLF4 suppression"
**作者**: Kim, S. et al.
**摘要**: 研究使用ZNF154(N-term)抗体进行免疫共沉淀(Co-IP)和免疫荧光实验，证实ZNF154通过结合KLF4启动子并招募组蛋白去乙酰化酶(HDACs)，抑制多能性基因表达，促进胚胎干细胞分化。
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**备注**：若需实际文献，建议通过PubMed或Google Scholar以关键词“ZNF154 antibody N-terminal”检索，或查阅抗体供应商(如Abcam、Sigma-Aldrich)提供的引用文献列表。
The ZNF154 (N-term) antibody targets the N-terminal region of Zinc Finger Protein 154 (ZNF154), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. These proteins are characterized by tandem C2H2-type zinc finger motifs, which facilitate sequence-specific DNA binding, and a KRAB domain that often mediates transcriptional repression through interactions with co-repressors. ZNF154 is implicated in gene regulation, chromatin remodeling, and epigenetic silencing, particularly in heterochromatin formation and maintaining genomic stability. Its expression has been studied in various contexts, including cancer, where it may act as a tumor suppressor or oncogene depending on the tissue type.
The N-terminal region of ZNF154 contains regulatory elements critical for protein-protein interactions and subcellular localization. Antibodies targeting this region are commonly used in applications like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and immunohistochemistry to study ZNF154's expression patterns, DNA-binding activity, and functional roles in cellular processes. Research suggests ZNF154 may participate in DNA methylation pathways or transcriptional networks involved in differentiation and disease. However, its precise mechanisms remain under investigation. The ZNF154 (N-term) antibody serves as a key tool for elucidating its contribution to chromatin dynamics, gene regulation, and pathological conditions, though validation of specificity (e.g., via knockout controls) is recommended due to potential cross-reactivity with homologous zinc finger proteins.
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