| WB | 1/100-1/4000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Gelsolin, AGEL, Actin-depolymerizing factor, ADF, Brevin, GSN |
| Entrez GeneID | 2934 |
| WB Predicted band size | 85.7kDa |
| Host/Isotype | Mouse IgM |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GSN antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 230-259 amino acids from the N-terminal region of human GSN. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GSN(N-term)抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*Gelsolin N-terminal phosphorylation regulates its interaction with actin*
**作者**：Smith A, et al.
**摘要**：研究聚焦于凝溶胶蛋白(GSN)N端结构域的磷酸化修饰对其与肌动蛋白结合能力的影响，通过特异性N端抗体验证了磷酸化位点突变对细胞骨架重塑的调控作用。
2. **文献名称**：*Development of a monoclonal antibody targeting gelsolin N-terminus for Alzheimer's disease biomarker detection*
**作者**：Chen L, et al.
**摘要**：报道了一种新型抗GSN N端单克隆抗体的开发，该抗体能够特异性识别阿尔茨海默病患者脑脊液中的GSN片段，为疾病早期诊断提供了潜在生物标志物检测工具。
3. **文献名称**：*N-terminal gelsolin antibody inhibits amyloid-β fibril formation in vitro*
**作者**：Wang Y, et al.
**摘要**：通过体外实验证明，针对GSN N端的抗体能够干扰β-淀粉样蛋白(Aβ)的纤维形成过程，提示其在神经退行性疾病治疗中的应用潜力。
4. **文献名称**：*Gelsolin N-terminal cleavage in cancer: Role of a custom antibody in tracking proteolytic fragments*
**作者**：Rodriguez M, et al.
**摘要**：利用定制的GSN N端抗体，揭示了肿瘤微环境中凝溶胶蛋白的酶切片段分布特征，并探讨其与肿瘤转移的相关性。
(注：上述文献为示例性内容，实际文献需通过PubMed/Google Scholar等数据库检索确认。)
GSN (N-term) antibody targets the N-terminal region of gelsolin, a multifunctional actin-binding protein critical for regulating cytoskeletal dynamics. Gelsolin plays a pivotal role in cell motility, apoptosis, and inflammation by severing, capping, or nucleating actin filaments in a calcium-dependent manner. It exists in cytoplasmic (80-90 kDa) and plasma (83 kDa) isoforms, derived from alternative splicing and post-translational modifications. The N-terminal region is essential for gelsolin’s actin-severing activity, as it contains the first gelsobactin homology domain (GH1) responsible for initial actin binding and conformational activation.
Antibodies against the N-terminus are widely used to study gelsolin’s expression, localization, and function in physiological and pathological contexts. They are employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate gelsolin’s role in diseases such as cancer (metastasis suppression), neurodegenerative disorders (e.g., amyloidosis in Alzheimer’s disease), and cardiovascular conditions (e.g., atrial fibrillation). Additionally, these antibodies help detect gelsolin cleavage products in apoptosis or pathological proteolysis. Specificity is often validated using knockout models or siRNA-mediated silencing. Research using GSN (N-term) antibodies has advanced understanding of cytoskeletal remodeling mechanisms and potential therapeutic targets in gelsolin-related pathologies.
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