| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LIM/homeobox protein Lhx2, Homeobox protein LH-2, LIM homeobox protein 2, LHX2, LH2 |
| Entrez GeneID | 9355 |
| WB Predicted band size | 44.4kDa |
| Host/Isotype | Mouse IgM |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This LHX2 monoclonal antibody is generated from mouse immunized with LHX2 recombinant protein. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LHX2抗体的参考文献示例(注：以下内容为假设性示例，实际文献需通过学术数据库核实)：
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1. **文献名称**：*"A Monoclonal Antibody Against LHX2 for Immunohistochemical Analysis in Mouse Brain Development"*
**作者**：Zhang Y. et al.
**摘要**：本研究开发了一种针对小鼠LHX2蛋白的单克隆抗体，验证了其在冷冻切片和石蜡包埋组织中的特异性。通过免疫组化分析，揭示了LHX2在胚胎期大脑皮层神经前体细胞中的动态表达模式，支持其在神经分化中的调控作用。
2. **文献名称**：*"LHX2 Antibody Validation and Application in Human Pluripotent Stem Cell-Derived Neurons"*
**作者**：Thompson R. et al.
**摘要**：文章系统验证了商业化LHX2抗体(货号：AB123.公司X)在流式细胞术和Western blot中的适用性，并利用该抗体证实LHX2在人诱导多能干细胞分化为谷氨酸能神经元过程中的关键作用。
3. **文献名称**：*"LHX2 in Hair Follicle Regeneration: Antibody-Based Localization and Functional Studies"*
**作者**：Chen L. et al.
**摘要**：通过免疫荧光染色(使用兔源LHX2多克隆抗体)，研究揭示了LHX2在毛囊干细胞维持中的空间表达特征，并利用抗体阻断实验证明LHX2通过Wnt信号通路调控再生过程。
4. **文献名称**：*"Cross-Species Reactivity of Anti-LHX2 Antibodies in Developmental Biology Research"*
**作者**：Kumar S. et al.
**摘要**：比较了多种LHX2抗体在小鼠、斑马鱼和人类组织中的交叉反应性，提出一种高特异性兔多克隆抗体的优化使用方案，为跨物种发育机制研究提供工具支持。
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如需真实文献，建议在PubMed或Google Scholar中检索关键词(如“LHX2 antibody validation”“LHX2 immunohistochemistry”)，并筛选近年发表的实验研究或方法学论文。
The LHX2 antibody is a crucial tool in studying the LIM-homeobox transcription factor 2 (LHX2), a protein encoded by the *LHX2* gene. LHX2 belongs to the LIM-homeodomain family, characterized by dual LIM domains that mediate protein interactions and a homeodomain for DNA binding. It plays critical roles in embryonic development, particularly in nervous system patterning, eye formation, and hematopoietic regulation. LHX2 is essential for maintaining neural progenitor cell identity and guiding cortical, hippocampal, and retinal development.
In research, LHX2 antibodies are primarily used for detecting LHX2 protein expression and localization via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help investigate LHX2's involvement in organogenesis, stem cell maintenance, and disease mechanisms. For example, LHX2 dysregulation is linked to glioblastoma, leukemia, and neurodevelopmental disorders. Commercial LHX2 antibodies are typically raised against specific epitopes (e.g., human or mouse LHX2 N-terminal regions) and validated for cross-reactivity in multiple species.
Recent studies also explore LHX2's role in cancer stemness and metastasis, making its antibody a valuable asset in oncology research. However, users must verify antibody specificity due to potential cross-reactivity with homologous LIM proteins. Proper controls, such as knockout validation, are recommended to ensure accurate experimental outcomes.
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