| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PAB-dependent poly(A)-specific ribonuclease subunit PAN3 {ECO:0000255|HAMAP-Rule:MF_03181}, PAB1P-dependent poly(A)-nuclease {ECO:0000255|HAMAP-Rule:MF_03181}, PAN deadenylation complex subunit 3 {ECO:0000255|HAMAP-Rule:MF_03181}, Pan3 |
| Entrez GeneID | 72587 |
| WB Predicted band size | 89.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Pan3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 727-755 amino acids from the C-terminal region of mouse Pan3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Pan3抗体的3篇参考文献及其摘要内容的简要概括：
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1. **文献名称**：*"PAN3 regulates somatic cell reprogramming through mRNA polyadenylation"*
**作者**：Chen Y, et al.
**摘要**：该研究利用特异性小鼠Pan3抗体，通过Western blot和免疫荧光技术，揭示了PAN3在体细胞重编程中的作用。研究表明，PAN3通过调控关键多能性基因的聚腺苷酸化影响重编程效率，抗体验证了Pan3敲除模型中蛋白表达的缺失。
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2. **文献名称**：*"Role of Pan3 in post-transcriptional regulation of hepatic lipid metabolism"*
**作者**：Kim S, et al.
**摘要**：作者使用小鼠Pan3抗体分析肝脏组织中PAN3蛋白的表达水平，发现其通过结合特定mRNA调控脂质代谢基因的稳定性。抗体在免疫共沉淀(Co-IP)实验中证实了PAN3与去腺苷酸化复合体的相互作用。
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3. **文献名称**：*"Characterization of a monoclonal antibody against mouse PAN3 for studying neuronal development"*
**作者**：Wang L, et al.
**摘要**：本文报道了一种高特异性小鼠Pan3单克隆抗体的开发与验证。通过免疫组化和流式细胞术，研究发现PAN3在小鼠大脑皮层神经元发育中动态表达，并参与突触形成相关mRNA的加工。
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**注**：以上文献为示例，实际引用时需根据真实发表的论文调整信息。建议通过PubMed或Web of Science以关键词“PAN3 antibody mouse”或“Pan3 murine immunolocalization”检索最新文献。
The Mouse Pan3 antibody is designed to detect the PAN3 homolog (Pan3) protein in murine models, a key component of the PAN2-PAN3 complex. This complex functions as a poly(A)-specific ribonuclease, critical for catalyzing mRNA deadenylation—the shortening of poly(A) tails—a process essential for regulating mRNA stability, translation efficiency, and degradation. PAN3 acts as a scaffold, coordinating interactions with deadenylases and other factors to fine-tune gene expression during cellular processes like development, stress responses, and cell cycle progression.
The antibody is typically generated in hosts such as rabbits or mice, targeting specific epitopes within conserved regions of the mouse Pan3 protein. It is validated for applications including Western blotting, immunoprecipitation, and immunofluorescence, often demonstrating high specificity through knockout (KO) validation. Researchers use this tool to study Pan3's role in mRNA metabolism, its interplay with other decay machinery components (e.g., CCR4-NOT complex), and its implications in diseases like cancer or neurodegeneration linked to dysregulated mRNA turnover. By enabling precise detection of Pan3 expression and localization, the antibody supports investigations into post-transcriptional regulatory mechanisms, offering insights into how deadenylation impacts cellular homeostasis and disease pathways.
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