| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase NIM1, NIM1 serine/threonine-protein kinase, Nim1k, Nim1 |
| Entrez GeneID | 245269 |
| WB Predicted band size | 49.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Nim1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-380 amino acids from the C-terminal region of mouse Nim1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Nim1抗体的模拟参考文献示例(请注意文献内容为虚构示例，供格式参考)：
1. **文献名称**: "Characterization of Nim1 Protein Expression in Murine Macrophages Using a Novel Monoclonal Antibody"
**作者**: Thompson, R. et al.
**摘要**: 本研究开发了一种针对小鼠Nim1蛋白的单克隆抗体，验证了其在流式细胞术和免疫荧光中的特异性。结果显示Nim1在激活的巨噬细胞中显著上调，提示其可能参与先天免疫反应调控。
2. **文献名称**: "Nim1 Antibody-Mediated Blockade Attenuates Autoimmune Encephalomyelitis in Mice"
**作者**: Sato, K. & Lee, M.
**摘要**: 通过使用小鼠Nim1中和抗体，研究者证明了抑制Nim1信号通路可减少实验性自身免疫性脑脊髓炎(EAE)模型的神经炎症和脱髓鞘，为多发性硬化症治疗提供潜在靶点。
3. **文献名称**: "Development of a Rabbit Anti-Mouse Nim1 Polyclonal Antibody for Immunohistochemical Analysis"
**作者**: Gonzalez, S. et al.
**摘要**: 该文献报道了一种兔源多克隆抗体的制备方法，验证了其在石蜡包埋组织切片中检测小鼠Nim1的特异性，并应用于脑组织中的蛋白定位研究，发现Nim1在小胶质细胞中高表达。
4. **文献名称**: "Nim1 Knockout Mice Phenotype Rescue Using Antibody-Based Targeted Therapy"
**作者**: Chen, W. et al.
**摘要**: 研究利用针对Nim1的功能性抗体，在Nim1基因敲除小鼠模型中成功恢复了部分免疫细胞功能，证实了Nim1在B细胞发育中的关键作用及抗体干预的潜在应用价值。
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**注意**：以上文献为模拟内容，实际研究中请通过PubMed、Google Scholar等平台以“Nim1 antibody mouse”或相关关键词检索真实文献。若“Nim1”为特定领域术语(如昆虫免疫蛋白Nimrod的同源物)，建议结合具体研究背景调整检索策略。
The Mouse Nim1 antibody, also known as anti-NEK1 (NimA-related kinase 1), targets the NEK1 protein, a serine/threonine kinase involved in cell cycle regulation, DNA damage response, and cilia function. NEK1 is a homolog of the *Aspergillus nidulans* NIMA kinase, critical for mitotic entry. In mammals, NEK1 plays dual roles in mitotic progression and maintaining genomic stability, with mutations linked to amyotrophic lateral sclerosis (ALS) and ciliopathies.
This antibody is typically generated by immunizing mice with recombinant NEK1 protein or specific epitopes, enabling detection of endogenous NEK1 in various applications. It is widely used in Western blotting, immunofluorescence, and immunoprecipitation to study NEK1’s localization, expression patterns, and interactions with proteins like HSP90 or components of the DNA repair machinery.
Research employing the Mouse Nim1 antibody has elucidated NEK1’s role in responding to ionizing radiation, regulating apoptosis, and mediating cilium assembly. Its dysfunction is associated with pathologies such as polycystic kidney disease and cancer. Validated in knockout (KO) models or siRNA knockdown controls, the antibody ensures specificity, making it a key tool for investigating NEK1’s molecular mechanisms in both physiological and disease contexts, particularly neurodegenerative and proliferative disorders.
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