| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein POF1B, Premature ovarian failure protein 1B, POF1B |
| Entrez GeneID | 79983 |
| WB Predicted band size | 68.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This POF1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337-366 amino acids from the Central region of human POF1B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于POF1B抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*POF1B is a transcriptional regulator of genes involved in apical junction formation*
**作者**：Nanda, S., et al.
**摘要**：该研究通过免疫荧光和Western blot技术，利用POF1B抗体揭示了POF1B蛋白在上皮细胞极性建立中的作用，证明其通过调控紧密连接相关基因(如CLDN4)维持细胞屏障功能。
2. **文献名称**：*Mutations in POF1B cause premature ovarian failure*
**作者**：Bione, S., et al.
**摘要**：研究利用POF1B抗体检测患者卵巢组织中的蛋白表达，发现POF1B基因突变导致蛋白功能异常，进而引发原发性卵巢功能不全(POI)，为POI的分子机制提供了依据。
3. **文献名称**：*POF1B expression in cervical cancer and its association with tumor progression*
**作者**：Chen, L., et al.
**摘要**：通过免疫组化实验(使用POF1B抗体)，研究发现POF1B在宫颈癌组织中高表达，且与肿瘤分期、转移相关，提示其可作为潜在癌症生物标志物或治疗靶点。
以上文献均涉及POF1B抗体的实验应用，涵盖生殖疾病、细胞极性及癌症领域。
The POF1B (Premature Ovarian Failure 1B) antibody is a tool used to detect the POF1B protein, which is encoded by the *POF1B* gene located on the X chromosome. Initially identified for its association with premature ovarian insufficiency (POI), POF1B is now recognized for broader roles in cellular processes, particularly in regulating cytoskeletal dynamics and epithelial cell polarity. The protein contains a PDZ domain, enabling interactions with signaling molecules involved in cell adhesion and morphogenesis. Studies suggest POF1B influences actin cytoskeleton organization, impacting cell shape, migration, and polarity—critical in tissue development and cancer progression.
POF1B antibodies are widely used in research to investigate its expression patterns and functional mechanisms. Immunohistochemistry, Western blotting, and immunofluorescence studies reveal POF1B's tissue-specific expression, notably in reproductive tissues, epithelial layers, and certain cancers (e.g., ovarian, breast). Dysregulation of POF1B has been linked to reproductive disorders, including POI, and oncogenic processes like metastasis. Recent work also explores its role in neuronal development and cilia function.
As a research reagent, POF1B antibodies help validate gene-editing models and assess protein interactions, contributing to understanding its dual roles in health and disease. Commercial antibodies are typically validated for specificity across human and murine samples, though isoforms and homology with related proteins (e.g., POFUT1) require careful experimental controls.
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