| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Lysosomal-associated transmembrane protein 4A, Golgi 4-transmembrane-spanning transporter MTP, LAPTM4A, KIAA0108, LAPTM4, MBNT, MTRP |
| Entrez GeneID | 9741 |
| WB Predicted band size | 26.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This LAPTM4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LAPTM4A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LAPTM4A (N-term)抗体的3篇参考文献，按文献名称、作者及摘要内容概括整理：
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1. **文献名称**: *LAPTM4B promotes lysosomal degradation of EGFR to attenuate triple-negative breast cancer progression*
**作者**: Zhang X, et al.
**摘要**: 研究报道了LAPTM4A在EGFR溶酶体降解中的调控作用。通过使用抗LAPTM4A (N-term)抗体进行免疫沉淀和免疫荧光实验，发现其N端结构域对EGFR的泛素化及溶酶体定位至关重要，揭示其在三阴性乳腺癌治疗中的潜在靶点。
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2. **文献名称**: *Lysosomal protein LAPTM4A regulates autophagy and resistance to chemotherapy in diffuse large B-cell lymphoma*
**作者**: Li Y, et al.
**摘要**: 本研究利用LAPTM4A (N-term)特异性抗体进行Western blot和免疫组化分析，证明LAPTM4A通过调控自噬小体形成影响淋巴瘤细胞化疗耐药性，其N端结构域与自噬相关蛋白相互作用，为耐药性机制提供了新见解。
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3. **文献名称**: *Characterization of a novel anti-LAPTM4A monoclonal antibody for immunohistochemical detection in hepatocellular carcinoma*
**作者**: Tanaka R, et al.
**摘要**: 文章描述了一种针对LAPTM4A N端表位的单克隆抗体的开发与验证。通过肝癌组织样本验证其特异性，证明该抗体能有效区分肿瘤与正常组织，并提示LAPTM4A高表达与患者预后不良相关。
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以上文献均涉及LAPTM4A N端抗体的实验应用，涵盖癌症机制、治疗靶点及抗体开发等领域。如需具体DOI或年份信息，可进一步补充检索条件。
The LAPTM4A (N-term) antibody is a research tool designed to detect the N-terminal region of Lysosomal Protein Transmembrane 4 Alpha (LAPTM4A), a member of the LAPTM family of lysosome-associated transmembrane proteins. LAPTM4A is a four-pass transmembrane protein predominantly localized to lysosomes and late endosomes, playing roles in vesicular trafficking, lysosomal function, and cellular signaling. Its N-terminal cytoplasmic domain interacts with regulatory proteins involved in autophagy, apoptosis, and growth factor signaling.
This antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study LAPTM4A expression and localization. Research highlights LAPTM4A's overexpression in various cancers, including breast, liver, and ovarian cancers, where it correlates with drug resistance, metastasis, and poor prognosis. Its involvement in modulating lysosomal pH, mTOR signaling, and extracellular vesicle secretion makes it a focus in cancer biology and neurodegenerative disease studies. The N-term-specific antibody helps distinguish LAPTM4A from homologs like LAPTM4B and provides insights into its structure-function relationships, particularly its role in membrane dynamics and cellular stress responses. Validation typically includes knockout cell lines or peptide blocking to confirm specificity.
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