| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Chondroitin sulfate synthase 1, Chondroitin glucuronyltransferase 1, Chondroitin synthase 1, ChSy-1, Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase 1, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1, N-acetylgalactosaminyltransferase 1, CHSY1, CHSY, CSS1, KIAA0990 |
| Entrez GeneID | 22856 |
| WB Predicted band size | 91.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CHSY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 504-533 amino acids from the Central region of human CHSY1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CHSY1抗体的示例参考文献格式(注：部分信息为模拟示例，建议通过学术数据库检索获取真实文献)：
1. **文献名称**: "CHSY1-mediated chondroitin sulfate synthesis is critical for cartilage development"
**作者**: Li X, et al.
**摘要**: 研究揭示了CHSY1在软骨素硫酸盐合成中的关键作用，利用特异性抗体证实了其在小鼠软骨发育中的表达模式，基因敲除导致骨骼畸形。
2. **文献名称**: "A novel monoclonal antibody recognizes human CHSY1 in tumor stromal cells"
**作者**: Tanaka K, et al.
**摘要**: 开发了针对人CHSY1的单克隆抗体，验证了其在肿瘤间质细胞中的高表达，提示CHSY1可能参与肿瘤微环境调控。
3. **文献名称**: "CHSY1 antibody-based detection of osteoarthritis biomarkers"
**作者**: Wang H, et al.
**摘要**: 采用CHSY1抗体建立ELISA检测方法，发现骨关节炎患者血清中CHSY1水平显著升高，提示其作为疾病生物标志物的潜力。
4. **文献名称**: "Characterization of CHSY1 knockout zebrafish using immunohistochemistry"
**作者**: Garcia S, et al.
**摘要**: 通过CHSY1抗体进行斑马鱼组织染色，发现基因缺失导致鳃弓发育异常，证实CHSY1在颅面形成中的作用。
**建议**：实际文献请通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar检索，使用关键词"CHSY1 antibody"+"应用领域(如cancer, cartilage等)"获取最新研究。部分抗体公司(如Abcam、CST)的产品说明书也会提供引用文献。
The CHSY1 antibody is a crucial tool for studying chondroitin sulfate synthase 1 (CHSY1), a glycosyltransferase involved in the biosynthesis of chondroitin sulfate (CS), a major component of extracellular matrices. CHSY1 catalyzes the polymerization of alternating glucuronic acid and N-acetylgalactosamine residues, forming CS chains critical for cell adhesion, signaling, and tissue development. Dysregulation of CHSY1 has been linked to skeletal disorders, such as Temtamy syndrome (a rare autosomal recessive condition characterized by craniofacial and limb abnormalities), and tumorigenesis, where altered CS expression influences metastasis and angiogenesis.
This antibody is commonly used in research to detect CHSY1 expression in tissues or cells via techniques like Western blotting, immunohistochemistry, or immunofluorescence. It aids in exploring CHSY1's role in developmental biology, cartilage formation, and disease mechanisms. Polyclonal or monoclonal CHSY1 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes within the protein. Validation often includes knockout cell lines or siRNA-mediated silencing to confirm specificity.
Recent studies highlight CHSY1's potential as a biomarker in cancers, including gliomas and colorectal cancer, where its overexpression correlates with poor prognosis. Researchers also utilize CHSY1 inhibitors or antibodies to modulate CS synthesis, offering insights into therapeutic strategies for CS-related pathologies. Proper antibody validation remains essential to ensure reliable data in both basic and translational studies.
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