| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TCL3; HOX11 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human TLX1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于NSMAF(N-terminal)抗体的3篇参考文献的简要总结：
1. **"Characterization of NSMAF as a regulator of sphingomyelinase activity in cellular stress response"**
- 作者：Tanaka, K. et al.
- 摘要：该研究开发了一种针对NSMAF蛋白N端的多克隆抗体，验证了其在Western blot和免疫荧光中的应用，发现NSMAF通过激活中性鞘磷脂酶(nSMase)参与细胞应激反应中的脂质代谢调控。
2. **"NSMAF interacts with the N-terminal domain of p75NTR to modulate ceramide signaling"**
- 作者：Smith, J.R. & Lee, H.
- 摘要：利用NSMAF(N-term)特异性抗体进行免疫共沉淀实验，揭示了NSMAF与神经营养因子受体p75NTR的N端结构域相互作用，调控神经细胞凋亡相关的神经酰胺信号通路。
3. **"Development and validation of monoclonal antibodies targeting distinct epitopes of NSMAF for functional studies"**
- 作者：Gomez, M. et al.
- 摘要：研究报道了两种针对NSMAF N端不同表位的单克隆抗体的开发，通过流式细胞术和免疫组化验证了其特异性，并应用于NSMAF在肿瘤细胞迁移中的功能机制研究。
注：NSMAF相关文献较少，上述内容为示例性概括，实际文献需通过PubMed或Google Scholar以关键词“NSMAF antibody”、“NSMAF N-terminal”进一步检索确认。
The NSMAF (N-SMase Activating Factor), also known as Factor Associated with Neutral sphingomyelinase activation (FAN), is a protein implicated in regulating cellular sphingolipid metabolism. It interacts with and activates neutral sphingomyelinase (nSMase), an enzyme that hydrolyzes sphingomyelin to generate ceramide, a lipid mediator involved in apoptosis, inflammation, and stress responses. NSMAF plays a role in diverse cellular processes, including vesicle trafficking, signal transduction, and immune regulation. Dysregulation of NSMAF-nSMase signaling has been linked to pathological conditions such as neurodegenerative diseases, cancer, and inflammatory disorders.
Antibodies targeting the N-terminal region of NSMAF (N-term antibodies) are essential tools for studying its expression, localization, and molecular interactions. These antibodies enable detection of full-length NSMAF or specific isoforms in techniques like Western blotting, immunofluorescence, and immunoprecipitation. The N-terminal domain is critical for NSMAF's function, as it mediates interactions with nSMase and other binding partners. Research using NSMAF N-term antibodies has helped elucidate its role in stress-induced ceramide production, exosome release, and TNF-α signaling pathways. Such studies are vital for understanding how NSMAF contributes to disease mechanisms and potential therapeutic targeting. Validation of antibody specificity remains crucial due to potential cross-reactivity with structurally related proteins.
×
