| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cas scaffolding protein family member 4, HEF-like protein, HEF1-EFS-p130Cas-like protein, HEPL, CASS4, C20orf32, HEFL |
| Entrez GeneID | 57091 |
| WB Predicted band size | 87.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CASS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610-639 amino acids from the C-terminal region of human CASS4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CASS4抗体的3篇参考文献示例(文献信息为假设性概括，实际引用请根据真实文献调整)：
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1. **标题**: *CASS4 promotes tumor cell invasion by regulating focal adhesion dynamics via its interaction with Crk*
**作者**: Smith A, et al.
**摘要**: 本研究通过Western blot和免疫荧光技术，利用CASS4特异性抗体揭示了其在乳腺癌细胞迁移中的作用。研究发现CASS4通过与Crk蛋白结合调控黏着斑周转，促进肿瘤细胞侵袭。抗体验证实验证实了CASS4在转移性癌细胞中的高表达。
2. **标题**: *Development and validation of a monoclonal antibody for CASS4 in non-small cell lung cancer diagnosis*
**作者**: Chen L, et al.
**摘要**: 文章报道了一种新型抗CASS4单克隆抗体的开发，并通过免疫组化验证其在非小细胞肺癌组织中的特异性表达。研究显示，CASS4抗体可作为潜在的诊断标志物，其表达水平与患者预后显著相关。
3. **标题**: *CASS4/HEPL modulates integrin signaling and suppresses colorectal cancer metastasis*
**作者**: Park JH, et al.
**摘要**: 通过shRNA敲低和CASS4抗体共免疫沉淀实验，作者发现CASS4通过整合素信号通路抑制结直肠癌转移。抗体介导的蛋白检测表明，CASS4缺失导致FAK/Src通路异常激活，增强癌细胞侵袭能力。
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如需具体文献，建议通过PubMed或Web of Science以“CASS4 antibody”、“CASS4 function”为关键词检索，并筛选涉及抗体应用的研究。
The CASS4 (Crk-associated substrate substrate family member 4) antibody is a tool used to study the CASS4 protein, a scaffolding adaptor protein encoded by the *CASS4* gene. CASS4 belongs to the Cas protein family, which plays critical roles in cell adhesion, migration, and signaling by interacting with focal adhesion kinases (FAK), integrins, and cytoskeletal components. Structurally, CASS4 contains conserved domains, including an SH3 domain, a substrate domain (SD), and multiple tyrosine phosphorylation sites, enabling it to mediate protein-protein interactions and downstream signaling cascades.
Research using CASS4 antibodies has highlighted its involvement in regulating cell motility, invasion, and cytoskeletal organization. In cancer biology, CASS4 expression has been linked to tumor progression and metastasis in various malignancies, such as breast and lung cancers, though its role appears context-dependent, acting as either an oncogene or tumor suppressor. Additionally, CASS4 has been implicated in neurological processes, including Alzheimer’s disease, due to its interaction with presenilin-1.
CASS4 antibodies are widely utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and post-translational modifications. These studies help elucidate CASS4’s functional dynamics in both physiological and pathological states, making it a target for therapeutic and diagnostic research.
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