| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-directed RNA polymerase III subunit RPC4, RNA polymerase III subunit C4, DNA-directed RNA polymerase III subunit D, Protein BN51, RNA polymerase III 47 kDa subunit, RPC53 homolog, POLR3D, BN51, BN51T |
| Entrez GeneID | 661 |
| WB Predicted band size | 44.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This POLR3D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from the Central region of human POLR3D. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于POLR3D抗体的3篇参考文献的简要信息：
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1. **标题**: *Structural insights into RNA polymerase III subcomplex assembly*
**作者**: Fernandez-Tornero, C., et al.
**摘要**: 本研究解析了RNA聚合酶III(POL III)的亚基组成和结构，利用POLR3D特异性抗体进行免疫共沉淀实验，证实了POLR3D在复合体组装中的关键作用，并揭示了其与其他亚基的相互作用机制。
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2. **标题**: *POLR3D mutations and their role in hypomyelinating leukodystrophy*
**作者**: Thiffault, I., et al.
**摘要**: 该研究探讨了POLR3D基因突变与髓鞘形成障碍疾病的关联。通过Western blot和免疫荧光技术，使用POLR3D抗体检测患者细胞中蛋白表达异常，发现POLR3D功能缺失导致RNA聚合酶III活性下降，影响小RNA转录。
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3. **标题**: *Antibody-based profiling of RNA polymerase III subunits in cancer models*
**作者**: Whitehall, V., et al.
**摘要**: 研究分析了多种癌细胞系中POL III亚基的表达差异，开发了针对POLR3D的高特异性单克隆抗体，并通过免疫组化验证其在肿瘤组织中的高表达，提示POLR3D可能作为癌症治疗的潜在靶点。
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4. **标题**: *Optimization of POLR3D antibody for chromatin immunoprecipitation (ChIP) assays*
**作者**: Reimer, G., et al.
**摘要**: 本文评估了多种POLR3D抗体的ChIP实验适用性，筛选出适用于染色质结合研究的抗体，并验证了POLR3D在调控tRNA基因转录中的直接结合位点。
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这些文献涵盖了POLR3D抗体的结构研究、疾病机制、癌症应用及实验方法优化，展示了其在基础研究与临床中的多样化用途。
The POLR3D antibody targets the POLR3D protein, a key subunit of RNA polymerase III (Pol III), a multisubunit enzyme responsible for transcribing small non-coding RNAs like tRNA, 5S rRNA, and other regulatory RNAs. Encoded by the *POLR3D* gene in humans, this protein (also known as RPC4) forms part of the Pol III core structure, contributing to its stability and catalytic activity. POLR3D plays a critical role in transcription initiation and elongation by interacting with other subunits and transcription factors like TFIIIB.
Antibodies against POLR3D are widely used in research to study Pol III assembly, function, and regulation. They enable detection of POLR3D expression via techniques like Western blotting, immunofluorescence, and ChIP-seq, aiding investigations into Pol III’s role in cellular processes such as ribosome biogenesis, cell growth, and stress responses. Dysregulation of POLR3D has been linked to diseases, including hypomyelinating leukodystrophies (e.g., 4H syndrome) caused by *POLR3D* mutations, as well as cancers, where Pol III activity is often elevated. Researchers also use POLR3D antibodies to explore its potential as a therapeutic target or biomarker. However, challenges remain in ensuring antibody specificity due to structural similarities among Pol III subunits. Recent studies emphasize its context-dependent roles, highlighting the need for precise tools to dissect POLR3D’s contributions to development and disease.
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