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Rabbit Polyclonal TLE6(N-term) Antibody

  • 中文名: TLE6 (N-term )抗体
  • 别    名: Transducin-like enhancer protein 6, TLE6
货号: IPDX31292
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesTransducin-like enhancer protein 6, TLE6
Entrez GeneID79816
WB Predicted band size63.5kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis TLE6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of human TLE6.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于 TLE6 (N-term) 抗体的 3 篇参考文献示例(注:文献为虚构示例,实际需根据具体数据库检索补充):

1. **文献名称**:*"TLE6 as a novel biomarker in ovarian cancer: Expression analysis using N-terminal specific antibodies"*

**作者**:Smith A, et al.

**摘要**:本研究利用针对 TLE6 N 端的特异性抗体,通过免疫组化(IHC)和蛋白质印迹(Western blot)技术,揭示了 TLE6 在卵巢癌组织中的高表达现象,并发现其与患者不良预后显著相关。

2. **文献名称**:*"Functional characterization of TLE6 in embryonic stem cell pluripotency via CRISPR/Cas9 and antibody-mediated protein localization"*

**作者**:Zhang L, et al.

**摘要**:通过 CRISPR/Cas9 敲除技术和 TLE6 N 端抗体的免疫荧光染色,研究者证实 TLE6 在维持小鼠胚胎干细胞多能性中起关键作用,并参与调控 Wnt 信号通路。

3. **文献名称**:*"Interactions between TLE6 and SMAD3 in TGF-β signaling pathway revealed by co-immunoprecipitation assays"*

**作者**:Johnson R, et al.

**摘要**:使用 TLE6 N 端抗体进行免疫共沉淀(Co-IP)实验,发现 TLE6 与 SMAD3 蛋白存在直接相互作用,为 TGF-β 信号通路的调控机制提供了新证据。

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**备注**:实际文献需通过 PubMed、Google Scholar 或抗体供应商(如 CST、Abcam)官网的引用列表检索,建议结合关键词 **"TLE6 antibody N-terminal"** 或 **"TLE6 immunolocalization"** 进一步筛选。

背景信息

The TLE6 (N-term) antibody is a tool used to detect the N-terminal region of the Transducin-like enhancer of split 6 (TLE6) protein, a member of the Groucho/TLE family. TLE proteins act as transcriptional co-repressors, interacting with transcription factors to regulate gene expression, particularly in developmental signaling pathways like Wnt and Notch. TLE6. specifically, is critical in early embryogenesis and cell fate determination, with roles in maintaining pluripotency and embryonic stem cell self-renewal. Unlike other TLE family members, TLE6 lacks the N-terminal Q-rich and GP domains but retains the C-terminal WD40 repeats for protein-protein interactions. The N-terminal region targeted by this antibody may include unique sequences that help differentiate TLE6 from other isoforms. Researchers use this antibody in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study TLE6 expression, localization, and interactions in models of development, cancer, or stem cell biology. Its specificity for the N-terminus makes it valuable for distinguishing TLE6 from truncated variants or degradation products. Validation typically involves knockout controls or peptide-blocking assays to confirm binding specificity.

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