| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ribonuclease 8, RNase 8, 3127-, RNASE8 |
| Entrez GeneID | 122665 |
| WB Predicted band size | 17.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RNASE8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-132 amino acids from the C-terminal region of human RNASE8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RNASE8抗体的模拟参考文献示例(请注意，这些文献为虚构示例，实际文献需通过学术数据库检索)：
1. **文献名称**: *Characterization of a novel polyclonal antibody against human RNASE8*
**作者**: Zhang L, et al.
**摘要**: 本研究开发了一种针对人源RNASE8的多克隆抗体，验证了其在Western blot和免疫组化中的特异性，并发现RNASE8在呼吸道黏膜上皮细胞中高表达，提示其可能在先天免疫中发挥作用。
2. **文献名称**: *RNASE8 expression and antibody-based detection in placental tissues*
**作者**: Gupta S, et al.
**摘要**: 通过免疫荧光和ELISA技术，首次报道RNASE8在胎盘滋养层细胞中的表达，其抗体可能作为妊娠相关感染的生物标志物研究工具。
3. **文献名称**: *Monoclonal antibody targeting RNASE8 inhibits bacterial growth in vitro*
**作者**: Tanaka K, et al.
**摘要**: 制备了靶向RNASE8功能结构域的单克隆抗体，证明其可通过阻断RNASE8的酶活性抑制革兰氏阴性菌生长，为抗感染治疗提供新思路。
4. **文献名称**: *Comparative analysis of RNASE8 antibody cross-reactivity across species*
**作者**: Müller R, et al.
**摘要**: 系统评估了商业RNASE8抗体在小鼠、恒河猴等模型中的交叉反应性，揭示了物种间RNASE8蛋白序列差异对抗体效价的影响。
**建议**：实际文献可通过PubMed/Google Scholar以"RNASE8 antibody"、"anti-RNASE8"为关键词检索，重点关注《PLOS ONE》《Journal of Immunology》等期刊近年发表的研究。
The RNASE8 antibody is designed to target ribonuclease 8 (RNASE8), a member of the RNase A superfamily, which includes enzymes involved in RNA metabolism, host defense, and tissue homeostasis. RNASE8 is a relatively understudied ribonuclease, initially identified through genomic analysis and later found to be expressed in specific tissues, such as the respiratory tract, male reproductive organs, and placenta. Its precise biological role remains unclear, though it is hypothesized to participate in innate immunity by degrading microbial RNA or modulating inflammatory responses, akin to other RNase A family members like RNASE7 or RNASE1 (angiotensinogenase).
Antibodies against RNASE8 are essential tools for investigating its expression patterns, localization, and functional mechanisms. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Studies using these antibodies may help clarify RNASE8’s role in mucosal immunity, reproductive health, or pathological conditions, such as infections or chronic inflammation. Commercial RNASE8 antibodies are typically developed in hosts like rabbits or mice, targeting specific epitopes through recombinant protein immunogens. Validation includes verifying reactivity with recombinant RNASE8 and ensuring minimal cross-reactivity with homologous RNases.
Current research on RNASE8 is limited, and available antibodies face challenges like low endogenous protein abundance and tissue-specific expression. Further work is needed to establish standardized assays and explore therapeutic or diagnostic applications.
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