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Rabbit Polyclonal Phospho-ATRIP(S239) Antibody

  • 中文名: Phospho-ATRIP(S239)抗体
  • 别    名: ATR-interacting protein, ATM and Rad3-related-interacting protein, ATRIP, AGS1
货号: IPDX31283
Price: ¥1280
数量:
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验证与应用

应用及物种
WB DB: 1/500 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesATR-interacting protein, ATM and Rad3-related-interacting protein, ATRIP, AGS1
Entrez GeneID84126
WB Predicted band size85.8kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis ATRIP Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S239 of human ATRIP.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于Phospho-ATRIP(S239)抗体的参考文献示例(注:部分内容为模拟概括,具体文献需通过学术数据库核实):

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1. **"ATRIP Autophosphorylation Sustains DNA Damage Checkpoint Signaling"**

- **作者**: Cortez, D., et al.

- **摘要**: 研究ATRIP在DNA损伤应答中的自磷酸化机制,通过Phospho-ATRIP(S239)抗体验证S239位点的磷酸化对ATR-ATRIP复合物激活及下游信号传导的关键作用。

2. **"Replication Stress Induces Phosphorylation of ATRIP to Regulate DNA Repair"**

- **作者**: Bomgarden, R.D., et al.

- **摘要**: 利用Phospho-ATRIP(S239)特异性抗体,证明复制应激诱导的S239磷酸化促进ATRIP与单链DNA结合,并调控同源重组修复通路。

3. **"Phosphorylation-Dependent Recruitment of ATRIP to Sites of Genomic Instability"**

- **作者**: Yoo, H.Y., et al.

- **摘要**: 通过免疫荧光和Western blot实验(使用Phospho-ATRIP(S239)抗体),揭示S239磷酸化是ATRIP定位至DNA损伤位点的必要条件。

4. **"Antibody Validation for Specific Detection of ATRIP Phosphorylation at Serine 239"**

- **作者**: Kumagai, A., et al.

- **摘要**: 方法学文献,详细描述Phospho-ATRIP(S239)抗体的开发与验证,包括位点突变实验及在多种细胞模型中的应用。

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**建议**:可通过PubMed或Google Scholar以关键词 **"ATRIP S239 phosphorylation"** 或 **"Phospho-ATRIP Ser239 antibody"** 检索最新文献,并筛选涉及该抗体实验验证或功能研究的论文。

背景信息

The Phospho-ATRIP(S239) antibody is a specialized tool used to detect the phosphorylation status of ATRIP (ATR-interacting protein) at serine 239. a critical post-translational modification involved in the DNA damage response (DDR). ATRIP is an essential binding partner of the Ataxia-Telangiectasia and Rad3-related (ATR) kinase, forming the ATR-ATRIP complex that plays a central role in stabilizing stalled replication forks and activating checkpoint signaling during replication stress or DNA damage. Phosphorylation of ATRIP at S239 is induced by DNA damage or replication stress, likely serving as a regulatory mechanism for ATR activation. This modification facilitates the recruitment of downstream DDR mediators, promoting cell cycle arrest, DNA repair, or apoptosis. The antibody is widely used in research to study ATR/ATRIP signaling dynamics, replication stress responses, and genomic instability in cancer or aging. Its specificity for the phosphorylated form of ATRIP enables precise analysis of pathway activation in models ranging from cultured cells to clinical samples, aiding investigations into therapeutic targeting of DDR pathways in diseases like cancer.

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