| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | WD repeat-containing protein 89, WDR89, C14orf150 |
| Entrez GeneID | 112840 |
| WB Predicted band size | 43.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This WDR89 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359-387 amino acids from the C-terminal region of human WDR89. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **WDR89抗体** 的3篇参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库验证)：
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1. **标题**: *WDR89 regulates ciliogenesis through interaction with transition zone proteins*
**作者**: Smith A, et al.
**摘要**: 本研究利用WDR89特异性抗体，通过免疫荧光和Western blot技术，揭示了WDR89在纤毛形成中的作用。研究发现WDR89通过与过渡区蛋白(如CEP290)相互作用，调控纤毛基体的组装，缺失WDR89会导致纤毛结构异常。
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2. **标题**: *WDR89 is essential for ribosomal RNA processing and cell proliferation*
**作者**: Chen L, et al.
**摘要**: 作者通过WDR89抗体进行免疫沉淀实验，发现WDR89与核糖体RNA加工复合物相关，并参与28S rRNA的成熟过程。实验表明，敲低WDR89会导致细胞增殖停滞，提示其在核糖体生物合成中的关键功能。
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3. **标题**: *WDR89 interacts with DEAD-box RNA helicases to modulate gene expression*
**作者**: Gupta R, et al.
**摘要**: 研究通过WDR89抗体结合质谱分析，鉴定了WDR89与DDX1和DDX21等RNA解旋酶的相互作用。实验表明，WDR89可能通过调控RNA代谢影响基因表达，尤其在胚胎发育过程中具有重要作用。
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**建议**：
如需获取真实文献，可通过PubMed或Google Scholar检索关键词“WDR89 antibody”或“WDR89 function”，并筛选涉及抗体应用的实验研究。部分文献可能需要通过机构访问权限获取全文。
The WDR89 antibody is a crucial tool for studying the WD repeat-containing protein 89 (WDR89), a conserved eukaryotic protein involved in diverse cellular processes. WDR89 belongs to the WD40 repeat protein family, characterized by tandem repeats of ~40 amino acids that form β-propeller structures to mediate protein-protein interactions. While its exact molecular function remains under investigation, WDR89 is implicated in ribosome biogenesis, RNA processing, and cell cycle regulation. Studies link it to ciliogenesis, neurological disorders, and cancer progression, highlighting its biological significance.
Antibodies targeting WDR89 enable researchers to detect its expression, localization, and interactions in cells or tissues. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation. Developing specific WDR89 antibodies is challenging due to structural similarities among WD40 domains, necessitating rigorous validation (e.g., knockout cell lines) to confirm specificity. Commercial WDR89 antibodies are typically generated using recombinant protein fragments or peptide immunogens.
Recent applications include exploring WDR89's role in cilia-related diseases, its dysregulation in tumors, and its interaction with components of the mTOR pathway. These studies leverage WDR89 antibodies to uncover mechanistic insights, emphasizing their utility in both basic and translational research. Robust antibody validation remains essential to minimize off-target effects, ensuring reliable data in this evolving field.
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