| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox1J; HomeoboxA13; HOX 1; HOX 1J; HOX A13; HOX1; HOX1J; HOXA 13; HOXA13; Transcription factor HOXA13;;HOX A13 |
| WB Predicted band size | Calculated MW: 40 kDa ; Observed MW: 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human HOX A13 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PGAM4 (N-term)抗体的参考文献示例(内容为假设性示例，建议通过科学数据库验证)：
1. **文献名称**：*"Testis-Specific Expression and Functional Characterization of PGAM4 in Mammalian Spermatogenesis"*
**作者**：Zhang Y, et al.
**摘要**：研究报道了PGAM4在小鼠睾丸中的特异性表达，利用N端抗体证实其在精子发生中的关键作用，并揭示其通过调控糖代谢影响生殖细胞发育。
2. **文献名称**：*"Development of a Novel Anti-PGAM4 Antibody Targeting the N-Terminal Domain for Cancer Biomarker Studies"*
**作者**：Tanaka K, et al.
**摘要**：研究团队开发了一种针对PGAM4 N端的多克隆抗体，验证其在多种癌症组织中的特异性识别能力，并探讨PGAM4作为前列腺癌生物标志物的潜力。
3. **文献名称**：*"Structural Insights into PGAM4 Isoform Specificity Using N-Terminal Epitope Mapping"*
**作者**：Smith R, et al.
**摘要**：通过N端抗体进行表位定位，揭示了PGAM4与其他PGAM家族成员的结构差异，强调了其在睾丸中的独特功能及进化保守性。
4. **文献名称**：*"PGAM4 Knockout Model Reveals Its Role in Cellular Energy Homeostasis via Glycolytic Regulation"*
**作者**：Chen L, et al.
**摘要**：利用N端抗体检测PGAM4在敲除模型中的表达缺失，证明其通过调节糖酵解通路维持生殖细胞能量稳态，并与男性不育相关。
建议通过PubMed、Google Scholar等平台，结合关键词“PGAM4 antibody N-terminal”或“PGAM4 spermatogenesis/cancer”检索最新文献以获取准确信息。
The PGAM4 (N-term) antibody is designed to detect the N-terminal region of Phosphoglycerate Mutase 4 (PGAM4), a tissue-specific isoform of the PGAM enzyme family. PGAMs are key glycolytic enzymes that catalyze the interconversion of 2- and 3-phosphoglycerate, regulating energy metabolism in cells. Unlike ubiquitously expressed isoforms (e.g., PGAM1/PGAM2), PGAM4 is testis-specific and thought to play a role in spermatogenesis due to its high expression in germ cells. The PGAM4 gene likely arose through retrotransposition of PGAM2 mRNA, resulting in a unique N-terminal sequence distinct from other isoforms.
The PGAM4 (N-term) antibody is commonly used in research to study reproductive biology, germ cell development, and metabolic adaptations in spermatogenic cells. Its specificity for the N-terminal region helps differentiate PGAM4 from other PGAM family members in applications like Western blotting, immunohistochemistry, and immunofluorescence. Studies using this antibody have contributed to understanding how tissue-specific metabolic enzyme variants support specialized cellular functions, particularly in male fertility. Aberrant PGAM4 expression has also been explored in contexts like testicular cancers, emphasizing its potential diagnostic relevance. Validation typically includes testing on testis tissue lysates or cell lines with confirmed PGAM4 expression.
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