| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Notchless protein homolog 1, NLE1 |
| Entrez GeneID | 54475 |
| WB Predicted band size | 53.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NLE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-372 amino acids from the C-terminal region of human NLE1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是假设性的参考文献示例，供参考。请注意，这些文献并非真实存在，建议通过学术数据库(如PubMed、Google Scholar)检索实际研究：
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1. **"NLE1 Antibody Reveals Its Role in Wnt Signaling Pathway Regulation"**
*Authors: Smith A, et al.*
摘要：本研究通过免疫共沉淀(Co-IP)和Western blot技术，利用NLE1抗体证实NLE1蛋白与β-catenin相互作用，调控Wnt信号通路活性，影响胚胎发育过程中的细胞分化。
2. **"NLE1 Expression Correlates with Colorectal Cancer Progression"**
*Authors: Zhang L, et al.*
摘要：通过免疫组化(IHC)分析结直肠癌组织样本，发现NLE1抗体标记的蛋白表达水平与肿瘤分期和患者预后显著相关，提示其作为潜在生物标志物的价值。
3. **"NLE1 Knockout Models Highlight Its Role in Neural Development"**
*Authors: Johnson R, et al.*
摘要：利用NLE1抗体在小鼠模型中验证基因敲除效果，发现NLE1缺失导致神经嵴细胞迁移异常，表明其对神经系统的早期发育具有关键作用。
4. **"NLE1 Antibody Applications in Diagnostic Assays"**
*Authors: Lee H, et al.*
摘要：评估NLE1抗体在ELISA和免疫荧光(IF)中的特异性与灵敏度，证明其在检测罕见遗传病相关NLE1突变中的诊断潜力。
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建议通过以下关键词检索真实文献：**NLE1 antibody applications**、**NLE1 protein function**、**NLE1 disease association**。
**Background of NLE1 Antibody**
The NLE1 (Notchless-like protein 1) antibody is a tool used to detect and study the NLE1 protein, a conserved eukaryotic protein implicated in critical cellular processes. NLE1 is part of the Wnt/Notch signaling pathways and plays a role in embryogenesis, cell differentiation, and proliferation. It interacts with components of the Notch signaling machinery, influencing γ-secretase activity and Notch receptor cleavage, which are essential for developmental patterning and tissue homeostasis.
Structurally, NLE1 contains WD40 repeat domains, enabling protein-protein interactions. Studies link NLE1 to ribosome biogenesis and cell cycle regulation, suggesting its broader role in cellular metabolism. Dysregulation of NLE1 has been associated with developmental disorders and cancer, making it a focus in biomedical research.
The NLE1 antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to localize and quantify NLE1 expression in tissues or cultured cells. It aids in exploring NLE1's function in developmental biology, cancer mechanisms, and potential therapeutic targeting. Commercial NLE1 antibodies are typically raised in rabbits or mice using recombinant protein fragments, with validation in specific experimental models. Ongoing research aims to clarify its precise molecular interactions and therapeutic relevance.
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