| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Phosphoinositide 3-kinase regulatory subunit 4, PI3-kinase regulatory subunit 4, PI3-kinase p150 subunit, Phosphoinositide 3-kinase adaptor protein, PIK3R4 |
| Entrez GeneID | 30849 |
| WB Predicted band size | 153.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PIK3R4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 819-847 amino acids from the Central region of human PIK3R4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与PIK3R4抗体相关的文献概览：
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1. **文献名称**: *PIK3R4 regulates cerebral development and mobility through vesicular trafficking*
**作者**: Smith A, et al. (2018)
**摘要**: 研究利用特异性PIK3R4抗体验证其在神经元中的亚细胞定位，发现PIK3R4通过调控自噬小体形成影响大脑发育，抗体Western blot显示PIK3R4敲除小鼠脑组织蛋白表达缺失。
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2. **文献名称**: *Vps15/PIK3R4 is essential for autophagy and lysosomal function in cancer cells*
**作者**: Chen L, et al. (2020)
**摘要**: 通过免疫荧光(使用兔源PIK3R4抗体)证明PIK3R4在癌细胞溶酶体膜上的定位，并揭示其通过结合Beclin-1调控自噬通路，抗体特异性经siRNA敲降实验验证。
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3. **文献名称**: *A novel role of PIK3R4 in insulin signaling revealed by antibody-based proteomic analysis*
**作者**: Kim J, et al. (2019)
**摘要**: 利用商业化PIK3R4抗体进行免疫沉淀，发现其与胰岛素受体底物(IRS1)相互作用，提示PIK3R4在代谢疾病中的潜在作用，抗体效价通过重组蛋白对照确认。
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**备注**：以上文献信息为示例性质，实际引用需核对真实出版物。建议通过PubMed或Google Scholar以“PIK3R4 antibody”或“Vps15 antibody”为关键词检索最新研究。
The PIK3R4 antibody is a research tool designed to detect and analyze the phosphoinositide-3-kinase regulatory subunit 4 (PIK3R4), a protein encoded by the PIK3R4 gene in humans. Also known as VPS15 (vacuolar protein sorting 15 homolog), PIK3R4 functions as a regulatory component of the class III phosphatidylinositol 3-kinase (PI3K) complex, which plays a critical role in intracellular vesicle trafficking, autophagy, and endosomal sorting. The PIK3R4 protein contains multiple domains, including a kinase-like domain and WD40 repeats, enabling interactions with other proteins like VPS34 (the catalytic subunit of the PI3K complex) to regulate lipid kinase activity.
Antibodies targeting PIK3R4 are widely used in biomedical research to study its expression, localization, and functional roles in cellular processes. They are commonly applied in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). These antibodies help elucidate PIK3R4’s involvement in diseases, particularly cancer and neurodegenerative disorders, where dysregulated autophagy or vesicle trafficking contributes to pathogenesis. For instance, altered PIK3R4 expression has been linked to tumor progression and chemoresistance. Researchers also utilize PIK3R4 antibodies to investigate its interaction with signaling pathways, such as mTOR, and its impact on lysosomal function. Commercial PIK3R4 antibodies are typically developed in hosts like rabbits or mice, validated for specificity using knockout controls or siRNA-mediated silencing, and optimized for various sample types (e.g., cell lysates, tissue sections). Their reliability is critical for advancing studies on autophagy-related mechanisms and therapeutic targeting.
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