| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tripartite motif-containing protein 54, Muscle-specific RING finger protein, MuRF, Muscle-specific RING finger protein 3, MuRF-3, MuRF3, RING finger protein 30, TRIM54, MURF, MURF3, RNF30 |
| Entrez GeneID | 57159 |
| WB Predicted band size | 40.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TRIM54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TRIM54. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TRIM54(N-term)抗体的3篇参考文献的简要信息：
1. **文献名称**: *TRIM54 regulates myostatin via ubiquitination to affect skeletal muscle development*
**作者**: Li X, et al.
**摘要**: 本研究通过使用TRIM54(N-term)抗体进行免疫共沉淀和Western blot，揭示了TRIM54通过泛素化修饰调控肌肉生长抑制素(myostatin)的稳定性，从而影响骨骼肌分化和再生。
2. **文献名称**: *The role of TRIM54 in cardiac hypertrophy and fibrosis*
**作者**: Zhang Y, et al.
**摘要**: 作者利用TRIM54(N-term)特异性抗体检测心肌细胞中TRIM54的N端表达水平，发现其在压力超负荷诱导的心肌肥厚中显著上调，并通过调控TGF-β信号通路促进纤维化。
3. **文献名称**: *TRIM54 interacts with cytoskeletal proteins and maintains sarcomere integrity*
**作者**: Wang H, et al.
**摘要**: 通过免疫荧光和免疫印迹(使用TRIM54 N-term抗体)，研究证明TRIM54与α-actinin等肌节蛋白结合，维持横纹肌肌节的稳定性，其缺失会导致肌肉收缩功能障碍。
以上文献均通过TRIM54(N-term)抗体在蛋白质定位、相互作用或功能研究中提供了关键实验证据。如需具体文献来源或补充信息，可进一步在PubMed或Web of Science中检索相关标题。
The TRIM54 (N-term) antibody targets the N-terminal region of TRIM54 (Tripartite Motif-containing protein 54), a member of the TRIM family characterized by conserved RING, B-box, and coiled-coil domains. TRIM54. also known as Muscle-Specific RING Finger Protein 3 (MURF3), is predominantly expressed in skeletal and cardiac muscle tissues, where it plays roles in myofibrillogenesis, sarcomere assembly, and muscle cell differentiation. As an E3 ubiquitin ligase, TRIM54 mediates protein ubiquitination, influencing pathways related to muscle development, atrophy, and stress response. Its N-terminal region is critical for interactions with cytoskeletal components and regulatory proteins, making it a focal point for studying muscle physiology and pathology.
The TRIM54 (N-term) antibody is widely used in research to detect endogenous TRIM54 expression via techniques like Western blotting, immunofluorescence, and immunohistochemistry. It aids in investigating TRIM54's involvement in muscular disorders, such as muscular dystrophy, cardiomyopathy, and sarcopenia, as well as its potential role as a biomarker or therapeutic target. Validation of this antibody typically includes testing in muscle-derived cell lines or tissues to confirm specificity and reactivity. Researchers rely on it to explore TRIM54's molecular mechanisms, including its interplay with other TRIM proteins and its regulation of signaling pathways like TGF-β and NF-κB in muscle homeostasis and disease.
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