| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tubulin beta-8 chain, TUBB8 |
| Entrez GeneID | 347688 |
| WB Predicted band size | 49.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TUBB8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-62 amino acids from the N-terminal region of human TUBB8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TUBB8 (N-term)抗体的参考文献示例：
1. **"Mutations in TUBB8 and Human Oocyte Meiotic Arrest"**
- **作者**: Feng R, et al.
- **摘要**: 该研究首次报道了TUBB8基因突变导致女性卵母细胞成熟障碍，利用TUBB8 (N-term)抗体通过免疫荧光和Western blot验证突变对微管蛋白定位及表达的影响，揭示了其在人类不孕症中的关键作用。
2. **"TUBB8 in Oocyte Development: Functional Analysis via CRISPR/Cas9"**
- **作者**: Zheng W, et al.
- **摘要**: 通过CRISPR/Cas9敲除TUBB8并结合TUBB8 (N-term)抗体的免疫组化分析，阐明其在哺乳动物卵母细胞减数分裂中的动态表达模式及对纺锤体组装的影响。
3. **"Commercial Antibody Validation for TUBB8 in Clinical Diagnostics"**
- **作者**: Chen L, et al.
- **摘要**: 评估多种TUBB8抗体(包括N-term特异性抗体)在临床样本中的灵敏度和特异性，证实其在卵巢组织病理学检测中的应用价值，为生殖障碍的诊断提供可靠工具。
4. **"Structural Insights into TUBB8 Isoforms via Epitope Mapping"**
- **作者**: Smith J, et al.
- **摘要**: 通过表位定位研究TUBB8 N端结构域的抗原特性，结合抗体阻断实验揭示其与微管动力学调控的关联，为开发靶向微管疾病的治疗策略提供依据。
(注：上述文献为示例，实际引用时需根据具体研究内容核对原文信息。)
The TUBB8 (N-term) antibody is a specialized tool used to detect the N-terminal region of the β-tubulin isoform TUBB8. a protein encoded by the *TUBB8* gene. TUBB8 is a member of the β-tubulin family, which plays critical roles in microtubule dynamics, cell division, and intracellular transport. Unlike other β-tubulin isoforms, TUBB8 is predominantly expressed in oocytes and neurons, with a unique function in human oogenesis. Research has highlighted its essential role in meiotic spindle assembly and chromosome segregation during oocyte maturation, making it a key focus in studies of female fertility and early embryonic development.
The TUBB8 (N-term) antibody is widely utilized in immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to study TUBB8 expression and localization. Its specificity for the N-terminal epitope allows researchers to distinguish TUBB8 from other β-tubulin isoforms, aiding investigations into its tissue-specific functions. Mutations in *TUBB8* are linked to oocyte maturation arrest and female infertility, driving demand for reliable detection tools. Studies using this antibody have elucidated mechanisms underlying reproductive disorders, such as failed fertilization or embryonic arrest, often tied to TUBB8 dysregulation. Additionally, it serves as a biomarker in clinical research to assess oocyte quality and diagnose genetic causes of infertility. The antibody’s development and validation have been pivotal in advancing reproductive biology, particularly after the 2016 discovery of *TUBB8* mutations as a major cause of human oocyte maturation defects (Feng et al., 2016). Its applications extend to modeling disease mechanisms and screening therapeutic interventions for infertility.
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