| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras-related protein Rab-3D, RAB3D, GOV, RAB16 |
| Entrez GeneID | 9545 |
| WB Predicted band size | 24.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RAB3D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-212 amino acids from the C-terminal region of human RAB3D. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RAB3D抗体的3篇参考文献及其摘要概括：
1. **"RAB3D regulates amylase secretion in pancreatic acinar cells via interaction with SNARE proteins"**
- **作者**: Tsuboi, T., Fukuda, M.
- **摘要**: 研究利用RAB3D抗体通过免疫印迹和免疫荧光技术，发现RAB3D通过调控SNARE蛋白的活性参与胰腺腺泡细胞的淀粉酶分泌过程，揭示了其在胞吐作用中的分子机制。
2. **"Adipocyte-specific deletion of Rab3D leads to adipose tissue dysfunction and metabolic disorders"**
- **作者**: Schlüter, O.M., et al.
- **摘要**: 通过RAB3D抗体进行组织特异性蛋白检测，发现脂肪细胞中RAB3D缺失会破坏脂联素囊泡运输，导致脂肪组织功能异常和胰岛素抵抗，表明其在代谢调控中的关键作用。
3. **"RAB3D as a biomarker for exosome secretion in breast cancer progression"**
- **作者**: Cazares, L.H., et al.
- **摘要**: 研究利用RAB3D抗体分析乳腺癌细胞外泌体，发现RAB3D高表达与肿瘤侵袭性相关，提示其可作为外泌体分泌的生物标志物及潜在治疗靶点。
注：上述文献为示例性概括，实际引用时建议通过PubMed或Web of Science等数据库核对具体信息。
Rab3D antibody is a research tool targeting the Rab3D protein, a member of the Rab GTPase family involved in regulating vesicle trafficking and exocytosis. Rab3D is primarily expressed in secretory cells, including pancreatic acinar cells, salivary glands, and adipose tissue, where it facilitates the transport and fusion of secretory vesicles with the plasma membrane. Unlike other Rab3 isoforms (e.g., Rab3A/B/C), Rab3D is uniquely associated with non-neuronal secretory pathways, particularly in specialized tissues requiring regulated secretion. Studies suggest its role in insulin granule release in pancreatic β-cells and lipid droplet dynamics in adipocytes, linking it to metabolic disorders like diabetes and obesity.
Rab3D antibodies are widely used in immunoblotting (Western blot), immunohistochemistry, and immunofluorescence to investigate protein expression, localization, and function. These antibodies help identify Rab3D's interaction partners, phosphorylation states, and tissue-specific distribution. Commercially available Rab3D antibodies are typically raised in rabbits or mice, targeting specific epitopes within conserved regions of the protein. Validation includes testing against knockout models or siRNA-treated cells to confirm specificity. Research utilizing Rab3D antibodies has advanced understanding of secretory mechanisms, membrane trafficking disorders, and metabolic dysregulation, making it a critical reagent in cell biology and endocrine research.
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