| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Poly(A) polymerase gamma, PAP-gamma, Neo-poly(A) polymerase, Neo-PAP, Polynucleotide adenylyltransferase gamma, SRP RNA 3'-adenylating enzyme, PAPOLG, PAP2, PAPG |
| Entrez GeneID | 64895 |
| WB Predicted band size | 82.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PAPOLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 685-714 amino acids from the C-terminal region of human PAPOLG. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及PAPOLG(Poly(A) polymerase gamma)抗体的参考文献及其摘要概括：
1. **文献名称**：PAPOLG variants impair polyadenylation in leukoencephalopathy
**作者**：Lardelli RM, et al. (2020)
**摘要**：该研究利用PAPOLG抗体进行蛋白质表达分析，发现基因突变导致PAPOLG功能缺失，引发脑白质病变。揭示了PAPOLG在RNA多聚腺苷酸化中的关键作用及其与神经退行性疾病的关联。
2. **文献名称**：Characterization of human poly(A) polymerase gamma family members
**作者**：Kashiwabara SI, et al. (2018)
**摘要**：通过PAPOLG抗体进行亚细胞定位实验，发现PAPOLG主要定位于细胞核，并参与线粒体RNA的转录后修饰，为解析其与线粒体功能紊乱相关疾病的机制提供了依据。
3. **文献名称**：CRISPR screen identifies PAPOLG as a therapeutic target in MYC-driven cancers
**作者**：Huang CH, et al. (2022)
**摘要**：研究使用PAPOLG抗体验证基因编辑后蛋白表达变化，发现抑制PAPOLG可通过破坏致癌RNA稳定性抑制MYC高表达肿瘤生长，提示其作为癌症治疗靶点的潜力。
注：上述文献为模拟示例，实际研究中建议通过PubMed或Web of Science以“PAPOLG antibody”或“Poly(A) polymerase gamma”为关键词检索最新文献。该靶点的功能研究目前多集中于RNA代谢异常相关疾病领域。
The PAPOLG antibody targets the PAPOLG protein, also known as poly(A) polymerase gamma (PAPγ) or terminal nucleotidyltransferase 5 (TENT5C), a member of the non-canonical poly(A) polymerase family. PAPOLG plays a critical role in RNA metabolism, particularly in cytoplasmic polyadenylation, which regulates mRNA stability, translation, and degradation. Unlike canonical poly(A) polymerases involved in nuclear mRNA processing, PAPOLG operates post-transcriptionally, influencing gene expression in response to cellular signals. It is implicated in immune responses, neurodevelopment, and cancer progression, with studies linking its dysfunction to diseases like multiple myeloma and neurodegenerative disorders.
The PAPOLG antibody is a key tool for detecting and quantifying PAPOLG expression in cells and tissues. Researchers use it in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study PAPOLG's localization, interaction partners, and regulatory mechanisms. Its development has advanced understanding of RNA-centric post-transcriptional regulation and opened avenues for therapeutic targeting. Commercial antibodies are typically validated for specificity, often through knockout controls, ensuring reliability in experimental models. As RNA biology gains prominence, the PAPOLG antibody remains vital for exploring dynamic mRNA modifications and their pathological implications.
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