关键信息
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基因名
cobB
- 应用
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别名
Regulatory protein SIR2 homolog
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种属
strain B100
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表达系统
E. coli
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标签
N- His-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
B0RM75
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表达区间
1-293aa
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分子量
48.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The study of cobB recombinant protein is situated in the context of understanding the biochemical pathways involved in bacterial metabolism and virulence. CobB, a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase, is crucial for the regulation of the cellular acetylation status, influencing various cellular processes, including gene expression and energy metabolism in bacteria. It is particularly notable in the model organism *Escherichia coli*, where it plays a role in the deacetylation of target proteins, thereby impacting their functions and interactions. Research has shown that CobB is involved in the regulation of virulence factors, suggesting its potential role in the pathogenicity of certain bacteria. Additionally, studying this enzyme can provide insights into its function in diverse physiological contexts, including stress responses and adaptation mechanisms. The recombinant production of CobB facilitates detailed characterization of its enzymatic activity and interaction with other cellular components. By generating CobB as a recombinant protein, researchers can explore its structure-function relationships, enzymatic kinetics, and inhibition, potentially paving the way for novel antibacterial strategies. Understanding the role of CobB in bacterial cells can contribute to broader applications in microbiology and biotechnology, offering avenues for developing treatments against bacterial infections or enhancing industrial fermentation processes.
