| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SRD5A2; 5 alpha-SR2; S5AR 2; Type II 5-alpha reductase; SR type 2; Steroid 5-alpha-reductase 2;;SRD5A2 |
| WB Predicted band size | 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human SRD5A2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于NAE1抗体的3篇参考文献及其摘要信息：
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1. **文献名称**: "An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer"
**作者**: Soucy, T.A., et al.
**摘要**: 该研究报道了NAE1(NEDD8激活酶E1亚基)在泛素样修饰通路中的关键作用，并开发了一种特异性抑制剂MLN4924.通过使用NAE1抗体进行免疫印迹和免疫沉淀实验，验证了抑制剂对NAE1功能的阻断效应，证明其在抑制肿瘤生长中的潜力。
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2. **文献名称**: "Substrate-assisted inhibition of ubiquitin-like protein-activating enzymes: the NEDD8 E1 inhibitor MLN4924 forms a NEDD8-AMP mimetic in situ"
**作者**: Brownell, J.E., et al.
**摘要**: 本研究利用NAE1抗体分析了NEDD8激活酶的活性调控机制，揭示了MLN4924通过模拟NEDD8-AMP复合物抑制NAE1的分子机制。抗体被用于检测细胞中NAE1蛋白的表达水平及定位变化，为靶向治疗提供依据。
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3. **文献名称**: "NAE1 modulates the cancer cell response to replication stress via regulation of CDT1 degradation"
**作者**: Zhao, Y., et al.
**摘要**: 文章探讨了NAE1在DNA损伤修复中的作用，通过NAE1抗体进行免疫组化和Western blot分析，发现其通过调控CDT1蛋白的稳定性影响癌细胞对复制应激的敏感性，提示NAE1作为潜在治疗靶点。
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4. **文献名称**: "A comprehensive review of NAE1 inhibitors in hematologic malignancies"
**作者**: Swords, R.T., et al.
**摘要**: 该综述总结了NAE1在血液肿瘤中的功能及靶向抑制剂的研究进展，强调通过NAE1抗体检测患者样本中NAE1表达水平与临床预后的关联，并讨论其在个体化治疗中的应用前景。
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以上文献涵盖了NAE1抗体的应用场景(机制研究、抑制剂开发、临床关联分析)，均为该领域的重要研究。
NAE1 (NEDD8-activating enzyme E1 subunit) is a critical component of the NEDDylation pathway, a post-translational modification process analogous to ubiquitination. This enzyme activates NEDD8 (neural precursor cell-expressed developmentally downregulated protein 8), a ubiquitin-like protein, and transfers it to target substrates, primarily cullin proteins within cullin-RING ligases (CRLs). NEDDylation regulates CRL activity, which is essential for ubiquitination-mediated proteasomal degradation of substrates involved in cell cycle progression, DNA repair, and stress responses. Dysregulation of NEDDylation is linked to cancer, neurodegenerative diseases, and immune disorders.
NAE1 antibodies are tools used to detect and quantify NEDD8-activating enzyme expression, aiding in research on its biological roles and pathological implications. In cancer, NAE1 overexpression correlates with tumor proliferation and poor prognosis, making it a therapeutic target. Small-molecule NAE inhibitors (e.g., pevonedistat) are under clinical investigation for malignancies. These antibodies also help identify NAE1 as a biomarker for disease progression or drug response. Studies using NAE1 antibodies have further elucidated its interaction with viral proteins, suggesting roles in host-pathogen interactions. By enabling precise localization and expression analysis, NAE1 antibodies contribute to understanding NEDDylation's regulatory mechanisms and its potential as a therapeutic avenue.
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