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Rabbit Polyclonal PLEKHM1(N-term) Antibody

  • 中文名： PLEKHM1 (N-term)抗体
  • 别    名： Pleckstrin homology domain-containing family M member 1, PH domain-containing family M member 1, 162 kDa adapter protein, AP162, PLEKHM1, KIAA0356
货号: IPDX31210
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesPleckstrin homology domain-containing family M member 1, PH domain-containing family M member 1, 162 kDa adapter protein, AP162, PLEKHM1, KIAA0356
Entrez GeneID9842
WB Predicted band size117.4kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis PLEKHM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids from the N-terminal region of human PLEKHM1.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于PLEKHM1 (N-term)抗体的3篇参考文献(虚拟示例，实际文献需查询数据库)：

1. **"PLEKHM1 regulates autophagosome-lysosome fusion through interaction with Rab7 and HOPS complex"**

- 作者：McKinnon C, et al.

- 摘要：研究PLEKHM1在自噬体-溶酶体融合中的作用，使用N端抗体通过免疫共沉淀验证其与Rab7及HOPS复合体的相互作用，并证实其功能缺失导致自噬流障碍。

2. **"N-terminal specific antibody reveals PLEKHM1 localization to lysosomes in osteoclasts"**

- 作者：Johansen T, et al.

- 摘要：利用N端抗体进行免疫荧光和Western blot分析，揭示PLEKHM1在破骨细胞溶酶体中的定位，并探讨其与骨吸收异常的关联。

3. **"PLEKHM1 deficiency disrupts lysosomal positioning and cellular homeostasis"**

- 作者：Marwaha R, et al.

- 摘要：通过N端抗体检测PLEKHM1敲除细胞模型，发现其缺失导致溶酶体运输异常，并影响细胞清除受损线粒体的能力。

(注：以上为模拟内容，实际文献需通过PubMed或SciHub等平台检索关键词“PLEKHM1 antibody”或“PLEKHM1 N-terminal”获取。)

背景信息

The PLEKHM1 (N-term) antibody is a research tool designed to detect the N-terminal region of the human PLEKHM1 protein (Pleckstrin Homology and RUN Domain Containing M1), a multifunctional adaptor protein involved in lysosomal trafficking, autophagy, and vesicle fusion processes. PLEKHM1 contains conserved domains, including a pleckstrin homology (PH) domain at its N-terminus, which facilitates interactions with membrane lipids and other proteins, and RUN domains that mediate binding to small GTPases like Rab7. These interactions position PLEKHM1 as a critical regulator of lysosome positioning, autophagosome-lysosome fusion, and osteoclast function. The N-terminal region targeted by this antibody is essential for its role in tethering lysosomes to the cytoskeleton and coordinating vesicle transport. Researchers use this antibody in applications such as Western blotting, immunofluorescence, and immunoprecipitation to study PLEKHM1 expression, localization, and molecular interactions in cellular models. Dysregulation of PLEKHM1 has been linked to osteopetrosis, neurodegenerative diseases, and cancer, making this antibody valuable for investigating disease mechanisms. Validation typically includes testing on knockout cell lines or tissues to confirm specificity for the N-terminal epitope. Its utility extends to studies on autophagy defects, lysosomal storage disorders, and bone remodeling pathways.

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