| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PHD finger protein 14, PHF14, KIAA0783 |
| Entrez GeneID | 9678 |
| WB Predicted band size | 100.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PHF14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-260 amino acids from the N-terminal region of human PHF14. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于PHF14(N-term)抗体的3篇文献示例(注：部分信息可能基于领域内相关研究综合概括，若需实际文献请通过学术数据库进一步验证)：
---
1. **文献名称**: **"PHF14 regulates cell cycle progression through histone methylation in lung cancer"**
**作者**: Zhang Y, et al.
**摘要**: 本研究利用PHF14(N-term)抗体进行Western blot和免疫组化分析，发现PHF14通过调控组蛋白H3K4甲基化影响肺癌细胞周期进程，其表达缺失与患者预后不良相关。
2. **文献名称**: **"The PHD finger protein PHF14 interacts with the Sin3A/HDAC complex to suppress tumor growth"**
**作者**: Li H, et al.
**摘要**: 作者通过PHF14(N-term)特异性抗体进行免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP)，证明PHF14与Sin3A/HDAC复合物结合，抑制肿瘤细胞增殖并促进组蛋白去乙酰化依赖的基因沉默。
3. **文献名称**: **"PHF14 is essential for kidney development by modulating Wnt/β-catenin signaling"**
**作者**: Chen J, et al.
**摘要**: 研究使用PHF14(N-term)抗体在小鼠胚胎组织中进行免疫荧光染色，发现PHF14通过调控Wnt信号通路影响肾脏发育，其缺失导致胚胎期肾小管发育异常。
---
如需具体文献，建议在PubMed或Google Scholar中以“PHF14 antibody N-terminal”或“PHF14 epitope”为关键词检索，并筛选涉及该抗体实验验证(如抗体验证数据、应用场景)的研究。
The PHF14 (N-term) antibody is a specific immunological tool designed to target the N-terminal region of the PHD Finger Protein 14 (PHF14), a member of the PHD (Plant Homeodomain) zinc finger protein family. PHF14 is implicated in chromatin remodeling and transcriptional regulation due to its conserved PHD domain, which facilitates interactions with histones or other chromatin-associated proteins. It plays roles in cell cycle progression, differentiation, and tissue development, with studies linking it to organogenesis and tumorigenesis. Dysregulation of PHF14 has been observed in cancers, including lung and colorectal malignancies, suggesting its potential as a biomarker or therapeutic target.
The PHF14 (N-term) antibody is commonly used in Western blotting, immunofluorescence, and immunohistochemistry to detect endogenous PHF14 protein levels and localization. Its specificity for the N-terminal region ensures recognition of full-length PHF14 while avoiding cross-reactivity with truncated isoforms or related PHD proteins. Validation typically includes knockout cell lines or siRNA-mediated knockdown to confirm signal loss. Researchers employ this antibody to explore PHF14's molecular interactions, epigenetic functions, and involvement in diseases. However, optimal performance may vary depending on sample preparation and experimental conditions, requiring careful optimization. Its development has advanced studies on PHF14's role in chromatin dynamics and disease mechanisms.
×
