| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 39S ribosomal protein L9, mitochondrial, L9mt, MRP-L9, MRPL9 |
| Entrez GeneID | 65005 |
| WB Predicted band size | 30.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MRPL9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-95 amino acids from the N-terminal region of human MRPL9. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MRPL9 (N-term)抗体的参考文献示例(注：以下内容为模拟虚构，仅供参考如何描述相关研究)：
1. **"Mitochondrial Ribosome Assembly: Role of MRPL9 in Subunit Stability"**
- **作者**: Smith A, et al.
- **摘要**: 本研究通过使用MRPL9 (N-term)抗体进行免疫印迹和免疫沉淀，揭示了MRPL9在线粒体大亚基组装中的关键作用，并发现其缺失导致核糖体结构异常。
2. **"MRPL9 Expression Correlates with Cellular Stress Response in Cancer"**
- **作者**: Lee B, et al.
- **摘要**: 利用MRPL9 (N-term)抗体的免疫组化分析，发现MRPL9在结直肠癌组织中高表达，且与氧化应激通路激活相关，提示其作为潜在治疗靶点。
3. **"Proteomic Profiling of Mitochondrial Dysfunction in Neurodegeneration"**
- **作者**: Chen C, et al.
- **摘要**: 通过MRPL9 (N-term)抗体的免疫荧光染色，证实MRPL9在阿尔茨海默病模型神经元线粒体中的定位异常，与能量代谢缺陷相关。
4. **"Functional Characterization of MRPL9 Knockout Models"**
- **作者**: Gonzalez D, et al.
- **摘要**: 研究使用MRPL9 (N-term)抗体验证基因敲除效率，发现MRPL9缺失导致线粒体翻译缺陷和胚胎发育异常。
**建议**：如需真实文献，可通过PubMed或Google Scholar搜索关键词“MRPL9 antibody”、“MRPL9 N-terminal”或“MRPL9 mitochondrial ribosome”筛选近期研究。
The MRPL9 (N-term) antibody is a monoclonal or polyclonal reagent designed to detect the N-terminal region of mitochondrial ribosomal protein L9 (MRPL9), a component of the mitochondrial large ribosomal subunit. MRPL9 plays a critical role in mitochondrial protein synthesis, which is essential for oxidative phosphorylation and cellular energy production. This antibody is commonly used in research to study mitochondrial ribosome assembly, function, or dysfunction linked to mitochondrial diseases, cancer, or metabolic disorders.
The N-terminal specificity ensures targeted recognition of full-length MRPL9 or its cleavage products, aiding in distinguishing isoforms or degradation fragments. Applications include Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, localization, or mitochondrial morphology in tissues or cultured cells. Validation typically involves knockdown/knockout controls or peptide blocking assays to confirm specificity.
Research using this antibody may explore MRPL9's role in mitochondrial translation, its interaction with other ribosomal proteins, or its involvement in pathologies like mitochondrial encephalopathies or chemotherapy resistance. Given the growing interest in mitochondrial biology, this tool contributes to understanding cellular metabolism, disease mechanisms, and potential therapeutic targets. Proper handling requires attention to mitochondrial extraction protocols to preserve protein integrity.
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