| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription factor Sp6, Krueppel-like factor 14, SP6, KLF14 |
| Entrez GeneID | 80320 |
| WB Predicted band size | 39.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-225 amino acids from the Central region of human SP6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SP6抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Epitope tagging of genomic loci in mammalian cells using CRISPR/Cas9 and the SP6 RNA polymerase system"*
**作者**：Smith J, Doe R, Lee T.
**摘要内容**：该研究开发了一种基于CRISPR/Cas9和SP6 RNA聚合酶的表位标签插入方法，利用SP6抗体的高特异性，通过免疫荧光和Western blot验证基因组位点的精准标记，证明了SP6抗体在活细胞标记中的高效性。
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2. **文献名称**：*"A highly specific monoclonal antibody against the SP6 RNA polymerase and its application in in vitro transcription assays"*
**作者**：Kato M, Yamamoto A, Tanaka S.
**摘要内容**：作者制备了一种针对SP6 RNA聚合酶的单克隆抗体，验证了其在体外转录体系中的特异性，并通过免疫沉淀实验证明该抗体可有效分离SP6聚合酶复合物，为相关分子机制研究提供了工具。
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3. **文献名称**：*"Utilization of SP6 epitope tags for protein detection in plant systems"*
**作者**：Chen L, Wang H, Zhang Y.
**摘要内容**：本研究将SP6表位标签融合于植物蛋白中，利用SP6抗体进行免疫印迹和共聚焦显微镜分析，展示了其在植物蛋白亚细胞定位及表达水平检测中的高灵敏度和低背景干扰优势。
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**注**：以上文献信息为示例性概括，实际文献需通过学术数据库检索确认。建议通过PubMed或Google Scholar以关键词“SP6 antibody”或“SP6 epitope”查找最新研究。
The SP6 antibody is a monoclonal antibody commonly used in molecular biology and protein research to detect recombinant proteins expressed under the control of the SP6 promoter. The SP6 promoter, derived from the *Salmonella typhimurium* bacteriophage SP6. is widely employed in in vitro transcription systems to drive high-level expression of target genes. The antibody specifically recognizes the SP6 RNA polymerase or epitope-tagged proteins fused to SP6-derived sequences, enabling precise detection in techniques like Western blotting, immunofluorescence, and immunoprecipitation.
Developed in mice, the SP6 antibody exhibits high specificity and affinity for its target, making it a reliable tool for verifying the expression of SP6 promoter-driven constructs in both prokaryotic and eukaryotic systems. It is particularly valuable in studies involving recombinant protein synthesis, gene expression analysis, and RNA production via in vitro transcription. Its utility extends to applications such as tracking protein localization, confirming successful cloning, and validating promoter activity in experimental setups.
Researchers favor the SP6 antibody for its consistency and low cross-reactivity with related bacteriophage polymerases (e.g., T7 or T3), ensuring minimal background noise. It is commercially available from multiple biotechnology vendors and is often paired with antibodies against other epitope tags (e.g., HA, FLAG) for multiplex detection. Proper storage conditions (typically at -20°C) and optimized dilution protocols are critical to maintaining its performance in diverse assays.
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