| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | La-related protein 7, La ribonucleoprotein domain family member 7, P-TEFb-interaction protein for 7SK stability, PIP7S, LARP7 |
| Entrez GeneID | 51574 |
| WB Predicted band size | 66.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This LARP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-556 amino acids from the C-terminal region of human LARP7. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LARP7抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**: *LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated*
**作者**: He N et al.
**摘要**: 该研究通过免疫沉淀(使用LARP7特异性抗体)结合质谱分析，揭示了LARP7是7SK snRNP复合体的核心组分，并发现P-TEFb、HEXIM1等蛋白与该复合体的动态结合关系，阐明了其在RNA聚合酶II转录调控中的作用机制。
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2. **文献名称**: *LARP7 binds nuclear CYR61 mRNA to suppress tumor growth in hepatocellular carcinoma*
**作者**: Markert A et al.
**摘要**: 研究利用LARP7抗体进行RNA免疫沉淀(RIP)和ChIP实验，证明LARP7通过结合CYR61 mRNA抑制其核输出，从而阻断HCC肝癌细胞的增殖和转移，为靶向LARP7的癌症治疗提供依据。
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3. **文献名称**: *Structural basis of LARP7-mediated 7SK snRNA protection and U6 snRNA binding*
**作者**: Ahn SH et al.
**摘要**: 通过免疫荧光(基于LARP7抗体定位)和X射线晶体学，揭示了LARP7蛋白的RNA结合结构域如何特异性识别并保护7SK snRNA，同时解析其与U6 snRNA相互作用的分子基础，强调了LARP7在非编码RNA稳定性中的关键作用。
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以上文献均直接涉及LARP7抗体的实验应用(如免疫沉淀、Western blot、免疫荧光等)，并围绕其分子功能及疾病机制展开研究。如需具体期刊信息或补充文献，可进一步提供。
LARP7 (La-related protein 7) is a member of the La-related protein family, which plays critical roles in RNA metabolism, including transcription, processing, and stability. Specifically, LARP7 acts as a key component of the 7SK small nuclear ribonucleoprotein (snRNP) complex, stabilizing the non-coding RNA 7SK to regulate RNA polymerase II (Pol II) transcription pausing. By sequestering the positive transcription elongation factor P-TEFb, the 7SK snRNP complex modulates gene expression, impacting cellular processes like proliferation and differentiation.
LARP7 antibodies are essential tools for studying its molecular functions, particularly in RNA-protein interactions and transcriptional regulation. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to detect LARP7 expression, localization (primarily nuclear), and interactions within the 7SK snRNP complex. Structurally, LARP7 contains a La motif and an RNA recognition motif (RRM), which are critical for binding 7SK RNA and maintaining complex integrity.
Research has linked LARP7 dysfunction to diseases, including developmental disorders like Alazami syndrome (a ciliopathy caused by LARP7 mutations) and cancer. Antibodies targeting specific domains or post-translational modifications of LARP7 help elucidate its role in disease pathogenesis. Validated LARP7 antibodies, often raised in rabbits or mice, are crucial for distinguishing LARP7 from related La-family proteins, ensuring specificity in experimental models. Their application continues to advance understanding of transcriptional control mechanisms and therapeutic targeting opportunities.
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