| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nucleoporin NUP53, 35 kDa nucleoporin, Mitotic phosphoprotein 44, MP-44, Nuclear pore complex protein Nup53, Nucleoporin Nup35, NUP35, MP44, NUP53 |
| Entrez GeneID | 129401 |
| WB Predicted band size | 34.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NUP35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 289-318 amino acids from the C-terminal region of human NUP35. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是基于NUP35相关研究的模拟参考文献示例(注：实际文献可能需要通过学术数据库查询)：
1. **"NUP35 regulates cell cycle progression by modulating nuclear transport"**
- 作者：Zhang L, et al.
- 摘要：研究通过NUP35抗体进行免疫荧光和Co-IP实验，发现NUP35通过调控特定蛋白的核质转运影响G1/S期转换，敲低NUP35导致细胞周期停滞。
2. **"Altered expression of NUP35 in colorectal cancer and its clinical significance"**
- 作者：Wang Y, et al.
- 摘要：使用NUP35抗体分析结直肠癌组织样本，发现NUP35表达与肿瘤分期和转移相关，提示其可能作为预后标志物。
3. **"Structural insights into NUP35 function within the nuclear pore complex"**
- 作者：Smith J, et al.
- 摘要：通过抗体介导的冷冻电镜技术解析NUP35在核孔复合体中的定位，揭示其与NUP93等蛋白互作以维持核孔稳定性。
4. **"NUP35 deficiency disrupts neuronal differentiation in vitro"**
- 作者：Chen R, et al.
- 摘要：利用NUP35抗体敲除模型，发现NUP35缺失导致神经干细胞分化异常，可能与核转运关键信号分子异常有关。
建议通过PubMed或Google Scholar以“NUP35 antibody”为关键词检索最新文献以获取准确信息。
The NUP35 antibody targets the NUP35 protein, a component of the nuclear pore complex (NPC), which regulates nucleocytoplasmic transport. NUP35. also known as NUP53. is part of the NPC's inner ring structure and plays a role in maintaining nuclear envelope integrity and mediating interactions between the NPC and nuclear membrane. It contributes to selective transport of macromolecules, linking the NPC to mitotic processes and chromatin organization.
Antibodies against NUP35 are essential tools for studying its localization, expression, and function in cellular dynamics. They are widely used in techniques like immunofluorescence, Western blotting, and immunoprecipitation to explore NPC assembly, nuclear transport mechanisms, and interactions with other nucleoporins. Research involving NUP35 antibodies has revealed its involvement in diseases, including cancers and cardiovascular disorders, where NPC dysfunction is implicated.
These antibodies are often validated using knockout cell lines or siRNA knockdown to confirm specificity. Commercial NUP35 antibodies are typically raised in rabbits or mice, with reactivity across human, mouse, and rat samples. Recent studies also utilize NUP35 antibodies to investigate its role in cellular stress responses and viral infection pathways, where NPC components are frequently hijacked. Proper controls, such as isotype-matched IgG and protein expression validation, are critical to ensure antibody reliability in experimental settings.
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