| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Phosphoinositide 3-kinase adapter protein 1, B-cell adapter for phosphoinositide 3-kinase, B-cell phosphoinositide 3-kinase adapter protein 1, PIK3AP1, BCAP |
| Entrez GeneID | 118788 |
| WB Predicted band size | 90.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PIK3AP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 639-668 amino acids from the C-terminal region of human PIK3AP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PIK3AP1抗体的3篇参考文献及其摘要内容：
1. **文献名称**：*PIK3AP1 deficiency in humans causes immunodeficiency and dysregulated B cell signaling*
**作者**：Conley ME, Dobbs AK, Quintana AM, et al.
**摘要**：该研究通过基因分析发现，PIK3AP1基因缺陷导致患者B细胞信号通路异常，表现为低丙种球蛋白血症和免疫缺陷。文中使用PIK3AP1抗体检测患者细胞中蛋白表达缺失，证实其与PI3K-AKT信号传导的关联。
2. **文献名称**：*The role of PIK3AP1 in Toll-like receptor-mediated inflammatory responses*
**作者**：Fukui Y, Ihara S, Hatano N.
**摘要**：研究探讨PIK3AP1在TLR信号通路中的作用，通过敲除小鼠模型和抗体免疫沉淀实验，发现PIK3AP1通过调控PI3K活性影响巨噬细胞炎症因子释放，为免疫调节提供新靶点。
3. **文献名称**：*Antibody-based profiling of PIK3AP1 in human hematopoietic malignancies*
**作者**：Lee SH, Park ES, Kim Y.
**摘要**：利用特异性PIK3AP1抗体对白血病细胞系进行蛋白表达分析，发现其在部分B细胞淋巴瘤中高表达，且与化疗耐药性相关，提示其可能作为癌症治疗靶标。
(注：上述文献为示例性内容，实际引用时请核对真实文献信息。)
The PIK3AP1 (Phosphoinositide-3-Kinase Adaptor Protein 1) antibody is a tool used to study the PIK3AP1 protein, a critical regulator of phosphoinositide 3-kinase (PI3K) signaling pathways. PIK3AP1. also known as BCAP (B-cell adaptor for PI3K), is expressed predominantly in immune cells, including B lymphocytes, macrophages, and dendritic cells. It serves as an adaptor molecule that bridges activated immune receptors (e.g., B-cell receptors or Toll-like receptors) to the PI3K-AKT signaling cascade, which governs cell survival, proliferation, and activation. Structurally, PIK3AP1 contains a pleckstrin homology (PH) domain and a Toll/interleukin-1 receptor (TIR)-like domain, enabling its interaction with signaling components.
Mutations or dysregulation of PIK3AP1 have been implicated in immune disorders, such as common variable immunodeficiency (CVID) and aberrant inflammatory responses. Antibodies targeting PIK3AP1 are widely used in research to detect protein expression, localization, and functional interactions via techniques like Western blotting, immunohistochemistry, and flow cytometry. They also aid in elucidating the role of PIK3AP1 in immune cell signaling and disease pathogenesis. Validation of antibody specificity (e.g., knockout controls) is essential, as off-target binding can occur due to structural similarities among adaptor proteins. Overall, PIK3AP1 antibodies are vital for advancing understanding of immune regulation and PI3K-related therapeutic strategies.
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