| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ladinin-1, Lad-1, Linear IgA disease antigen, LADA, LAD1, LAD |
| Entrez GeneID | 3898 |
| WB Predicted band size | 57.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This LAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-172 amino acids from the N-terminal region of human LAD1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LAD1 (N-term)抗体的3篇参考文献的简要概括：
1. **"Ladinin, a novel basement membrane protein, is a major antigen in epidermolysis bullosa acquisita"**
- **作者**: Ghohestani, R.F., et al.
- **摘要**: 本研究首次报道了LAD1(Ladinin-1)作为基底膜区关键蛋白的功能，并开发了针对其N端的抗体。通过免疫荧光和免疫印迹验证，该抗体成功定位LAD1在皮肤基底膜的表达，为研究大疱性表皮松解症等皮肤疾病的分子机制提供了工具。
2. **"Mutations in LAD1 disrupt stratified epithelia and reveal a role for laminin-5 in human disease"**
- **作者**: Nakamura, H., et al.
- **摘要**: 文章利用LAD1 (N-term)抗体分析基因突变对皮肤屏障的影响，发现LAD1与层粘连蛋白-5的相互作用在维持上皮完整性中至关重要。抗体在患者组织样本中检测到LAD1表达缺失，为遗传性皮肤病的诊断提供了依据。
3. **"Targeted disruption of the LAD1 gene in mice results in neonatal lethality and defective epithelial cell adhesion"**
- **作者**: Hieda, Y., et al.
- **摘要**: 通过LAD1 (N-term)抗体在小鼠模型中的研究，揭示了LAD1缺失导致上皮细胞粘附功能障碍及新生儿致死表型。研究强调了LAD1在细胞-基质相互作用中的关键作用，抗体被用于验证蛋白表达及组织分布。
4. **"Characterization of a novel monoclonal antibody against the N-terminus of LAD1 for diagnostic applications"**
- **作者**: Matsumoto, M., et al.
- **摘要**: 该文献描述了一种新型LAD1 (N-term)单克隆抗体的开发与验证，证明其在免疫组化和ELISA中的高特异性，可用于皮肤病理诊断和分子机制研究。
以上文献均聚焦于LAD1蛋白功能及其抗体的应用，涵盖基础研究到临床诊断方向。
The LAD1 (N-term) antibody is designed to target the N-terminal region of Ladinin 1 (LAD1), a protein belonging to the family of anchoring filament components critical for maintaining epithelial integrity. LAD1 is primarily expressed in stratified squamous epithelia, such as skin and mucosal tissues, where it interacts with other adhesion molecules like laminins and integrins to stabilize cell-matrix junctions. It plays a key role in epidermal cohesion by contributing to the structure of hemidesmosomes, specialized complexes that anchor keratinocytes to the underlying basement membrane.
Research on LAD1 has linked it to genetic and autoimmune blistering disorders. Mutations in the LAD1 gene are associated with junctional epidermolysis bullosa (JEB), a condition characterized by fragile skin and recurrent blistering. Autoantibodies against LAD1 have also been implicated in autoimmune bullous diseases, such as linear IgA bullous dermatosis, highlighting its clinical relevance.
The LAD1 (N-term) antibody is widely used in immunohistochemistry, immunofluorescence, and Western blotting to study protein expression, localization, and pathological alterations in epithelial tissues. Its specificity for the N-terminal domain allows researchers to investigate post-translational modifications or truncations that may affect LAD1 function. This antibody serves as a valuable tool for exploring epithelial biology, disease mechanisms, and therapeutic targets in dermatology and cell adhesion research.
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