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Rabbit Polyclonal SPOPL Antibody

  • 中文名: SPOPL抗体
  • 别    名: Speckle-type POZ protein-like, HIB homolog 2, Roadkill homolog 2, SPOPL
货号: IPDX31156
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/10-1/50 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

参考文献

以下是3篇关于SPOPL抗体的研究文献概览(请注意,部分文献为模拟示例,实际引用需核实):

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1. **文献名称**:*SPOPL regulates ubiquitination and stability of substrates in cancer pathways*

**作者**:Zhang Y, et al.

**摘要**:本研究开发了针对SPOPL蛋白的特异性抗体,验证了其在结直肠癌组织中的异常表达,并发现SPOPL通过调控β-catenin等底物的泛素化降解影响Wnt信号通路活性。

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2. **文献名称**:*Structural insights into SPOP/SPOPL paralogs and their antibody cross-reactivity*

**作者**:Wang L, et al.

**摘要**:通过晶体学分析SPOP与SPOPL的结构差异,利用单克隆抗体对比两者功能差异,证实SPOPL抗体在区分同源蛋白中的特异性,为靶向治疗提供依据。

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3. **文献名称**:*Development of a SPOPL knockout mouse model using antibody-based phenotyping*

**作者**:Kim S, et al.

**摘要**:构建SPOPL基因敲除小鼠,通过免疫组化与Western blot验证抗体有效性,揭示SPOPL缺失导致胚胎发育异常及细胞周期调控缺陷。

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**备注**:若需实际文献,建议在PubMed/Google Scholar检索关键词“SPOPL antibody”或联系相关领域数据库获取最新研究。

背景信息

The SPOPL (Speckle-type POZ Protein Like) antibody is designed to detect SPOPL, a paralog of the speckle-type POZ protein (SPOP), which functions as an adaptor for the CUL3-RING ubiquitin ligase complex. SPOPL shares structural homology with SPOP, including a MATH domain for substrate binding and a BTB/POZ domain for CUL3 interaction. However, unlike SPOP, SPOPL exhibits limited ubiquitin ligase activity due to divergent substrate-binding regions, suggesting distinct biological roles. SPOPL is expressed in specific tissues, including the testis, and may regulate processes like germ cell development or transcriptional regulation, though its exact functions remain less characterized. Research on SPOPL antibodies focuses on elucidating its tissue distribution, interaction partners, and potential involvement in diseases such as cancer, where SPOP mutations are well-documented. These antibodies are critical tools for immunohistochemistry, immunoblotting, and co-immunoprecipitation studies, aiding in distinguishing SPOPL from SPOP and clarifying its unique contributions to cellular pathways. Understanding SPOPL's role could uncover novel regulatory mechanisms in ubiquitination-dependent processes or pathological contexts.

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