| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription elongation factor B polypeptide 3, Elongin 110 kDa subunit, Elongin-A, EloA, RNA polymerase II transcription factor SIII subunit A1, SIII p110, TCEB3 |
| Entrez GeneID | 6924 |
| WB Predicted band size | 89.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Elongin A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-127 amino acids from the N-terminal region of human Elongin A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与Elongin A (N-terminal)抗体相关的文献摘要概览：
1. **"Elongin A complex mediates transcriptional elongation through RNA polymerase II"**
*Authors: Aso T., et al.*
摘要：研究揭示了Elongin A作为转录延伸复合体的核心组分，其N端结构域在结合RNA聚合酶II中的关键作用。文中通过特异性N端抗体验证了Elongin A在染色质免疫沉淀(ChIP)实验中的功能定位。
2. **"Structural basis for the interaction of Elongin A with the von Hippel-Lindau tumor suppressor protein"**
*Authors: Stebbins C.E., et al.*
摘要：通过X射线晶体学解析了Elongin A N端结构域与VHL蛋白的相互作用机制。研究中利用N端抗体进行免疫共沉淀实验，证实了该区域在肿瘤抑制通路中的分子对接功能。
3. **"Regulation of Elongin A ubiquitination and stability by the SOCS-box motif"**
*Authors: Kamura T., et al.*
摘要：探讨Elongin A的蛋白稳定性调控机制，发现其N端结构域通过SOCS-box介导泛素化降解。Western blot实验中采用N端特异性抗体验证了不同突变体对蛋白稳定性的影响。
注：上述文献为示例性概括，实际引用需根据具体实验背景补充完整信息(期刊、年份及DOI)。建议通过PubMed或Google Scholar以关键词“Elongin A N-terminal antibody”或“TCEB3 antibody”检索最新研究。
Elongin A (N-term) antibody targets the N-terminal region of Elongin A, a critical subunit of the Elongin complex involved in transcriptional regulation and RNA polymerase II (Pol II)-mediated transcription elongation. Elongin A (also known as transcription elongation factor B subunit 1. TCEB1) interacts with Elongin B and C to form a heterotrimeric complex that enhances the processivity of RNA Pol II by suppressing transient pausing during transcription. The N-terminal domain of Elongin A plays a key role in binding to the phosphorylated C-terminal domain (CTD) of RNA Pol II, facilitating recruitment of the complex to transcription sites. This interaction is essential for coordinating transcription elongation with chromatin remodeling, mRNA processing, and DNA repair.
Antibodies specific to the N-terminus of Elongin A are widely used in research to study its expression, localization, and functional interactions. They enable detection of endogenous Elongin A in techniques such as Western blotting, immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP). These antibodies help investigate Elongin A’s regulatory roles in cellular processes, including its involvement in the hypoxia response pathway via association with the von Hippel-Lindau (VHL) tumor suppressor complex. Dysregulation of Elongin A has been implicated in cancers and other diseases, making its N-terminal antibodies valuable tools for exploring molecular mechanisms in both normal and pathological states. Specificity for the N-terminal region ensures recognition of full-length, functionally active Elongin A, avoiding cross-reactivity with truncated isoforms or degradation products.
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