| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras-related protein Rab-27B, C25KG, RAB27B |
| Entrez GeneID | 5874 |
| WB Predicted band size | 24.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RAB27B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-150 amino acids from the Central region of human RAB27B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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The RAB27B antibody is a crucial tool for studying the RAB27B protein, a member of the RAB GTPase family involved in regulating intracellular vesicle trafficking and exocytosis. RAB27B plays a key role in secretory pathways, particularly in the transport and release of specialized vesicles, such as melanosomes, secretory granules in endocrine cells, and exosomes. It interacts with effector proteins like Slp4-a and MyRIP to mediate membrane docking and fusion processes. Dysregulation of RAB27B is linked to pathological conditions, including Griscelli syndrome type 2 (when combined with MYO5A mutations), cancer metastasis (via exosome-mediated communication), and immune disorders.
RAB27B antibodies, typically raised in rabbits or mice, enable the detection and localization of endogenous RAB27B in techniques like Western blotting, immunofluorescence, and immunohistochemistry. These antibodies help elucidate its expression patterns, subcellular distribution (e.g., association with late endosomes/lysosomes), and functional interactions in diverse cell types. Validated antibodies are essential for distinguishing RAB27B from its homolog RAB27A, which shares 71% amino acid identity but has distinct roles. Research using these antibodies has advanced understanding of RAB27B’s involvement in tumor microenvironment modulation, platelet secretion, and neurological processes, highlighting its therapeutic potential.
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