| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mediator of RNA polymerase II transcription subunit 22, Mediator complex subunit 22, Surfeit locus protein 5, Surf-5, MED22, SURF5 |
| Entrez GeneID | 6837 |
| WB Predicted band size | 22.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MED22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MED22. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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MED22 (N-term) antibody is a tool designed to target the N-terminal region of the MED22 protein, a subunit of the Mediator complex—a conserved multi-protein assembly critical for regulating RNA polymerase II (Pol II)-dependent transcription in eukaryotes. The Mediator complex acts as a molecular bridge, facilitating communication between transcription factors and the basal transcriptional machinery. MED22. also known as Mediator of RNA Polymerase II Subunit 22. contributes to the structural and functional integrity of the complex, playing roles in transcriptional initiation, elongation, and chromatin remodeling.
The antibody is commonly used in research applications such as Western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) to detect endogenous MED22 expression. Its specificity for the N-terminal region ensures recognition of full-length MED22 or relevant cleavage products, aiding studies on protein localization, interaction partners, and expression levels under various conditions. Validation often includes testing in knockout or knockdown models to confirm absence of cross-reactivity.
MED22 dysregulation has been implicated in diseases like cancer and developmental disorders, making this antibody valuable for exploring transcriptional mechanisms in both normal physiology and pathology. Researchers use it to dissect Mediator complex dynamics, gene regulation networks, and responses to cellular signals, providing insights into therapeutic targets. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) vary by commercial source, impacting experimental design and reproducibility.
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