| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tubulin polyglutamylase TTLL4, 6---, Tubulin--tyrosine ligase-like protein 4, TTLL4, KIAA0173 |
| Entrez GeneID | 9654 |
| WB Predicted band size | 133.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TTLL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 464-493 amino acids from the Central region of human TTLL4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TTLL4抗体的3篇虚构参考文献示例(实际文献需通过数据库验证)：
1. **文献名称**：*TTLL4-Mediated Tubulin Modification Regulates Cancer Cell Metastasis*
**作者**：Zhang Y, et al.
**摘要**：本研究利用特异性TTLL4抗体，揭示了TTLL4在微管酪氨酸化修饰中的作用，并发现其过表达促进乳腺癌细胞迁移。通过免疫沉淀和免疫荧光技术，证实TTLL4与微管动力学调控直接相关。
2. **文献名称**：*Development of a Monoclonal Antibody Against TTLL4 for Neurological Disease Research*
**作者**：Smith J, et al.
**摘要**：报道一种新型TTLL4单克隆抗体的开发与验证，通过Western blot和免疫组化证明其在小鼠脑组织中的特异性。研究发现TTLL4在阿尔茨海默病模型中表达异常，提示其潜在病理作用。
3. **文献名称**：*TTLL4 Knockdown Impairs Cilia Formation via Antibody-Based Functional Screening*
**作者**：Chen L, et al.
**摘要**：通过CRISPR-Cas9和TTLL4抗体功能筛选，证明TTLL4缺失导致纤毛形成缺陷。研究结合流式细胞术和共聚焦显微镜，阐明TTLL4在纤毛组装中的关键调控机制。
(注：以上为模拟内容，实际文献请通过PubMed、Google Scholar等平台检索。)
TTLL4 (tubulin tyrosine ligase-like 4) is a member of the TTLL family of enzymes involved in post-translational modifications of tubulin, particularly the addition of glutamate residues to microtubules (a process called glutamylation). This modification regulates microtubule stability, dynamics, and interactions with motor proteins, impacting cellular processes like cilia function, cell division, and neuronal development. TTLL4 is specifically implicated in initiating primary glutamylation, influencing microtubule network organization.
Antibodies targeting TTLL4 are essential tools for studying its expression, localization, and function. They are used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect TTLL4 in tissues or cultured cells. Research has linked TTLL4 dysregulation to diseases, including cancers (e.g., colorectal cancer, glioblastoma) and ciliopathies, highlighting its potential as a therapeutic target or biomarker.
Commercial TTLL4 antibodies are typically raised against specific epitopes (e.g., N-terminal or C-terminal regions) and validated for specificity using knockout controls or siRNA-mediated knockdown. Challenges include cross-reactivity with other TTLL family members due to structural homology, emphasizing the need for rigorous validation. Recent studies also explore TTLL4's role in immune responses and neurodegenerative disorders, expanding its biomedical relevance.
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