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Rabbit Polyclonal WDR3(N-term) Antibody

  • 中文名: WDR3 (N-term)抗体
  • 别    名: WD repeat-containing protein 3, WDR3
货号: IPDX31102
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/10-1/50 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesWD repeat-containing protein 3, WDR3
Entrez GeneID10885
WB Predicted band size106.1kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenThis WDR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-236 amino acids from the N-terminal region of human WDR3.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于WDR3 (N-term)抗体的3篇参考文献示例(基于公开文献信息概括,部分为模拟内容):

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1. **文献名称**:*WD repeat domain 3 (WDR3) is essential for ribosomal RNA processing and cell proliferation*

**作者**:Lin Y, et al.

**摘要**:该研究通过使用针对WDR3 N端的特异性抗体,揭示了WDR3在核糖体RNA前体加工中的关键作用。实验表明,WDR3缺失会导致rRNA成熟障碍和细胞周期停滞,抗体在Western blot和免疫荧光实验中验证了WDR3的亚细胞定位及表达水平变化。

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2. **文献名称**:*A novel role of WDR3 in regulating p53 stability and tumor suppression*

**作者**:Chen J, et al.

**摘要**:研究利用WDR3 (N-term)抗体进行免疫共沉淀(Co-IP)实验,发现WDR3通过结合去泛素化酶USP7调控p53蛋白稳定性。抗体验证了WDR3在多种癌症组织中的表达异常,并证明其与患者预后相关。

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3. **文献名称**:*Structural and functional analysis of WDR3 in the human RNA exosome complex*

**作者**:Smith R, et al.

**摘要**:通过WDR3 N端抗体的表位定位,结合冷冻电镜技术,解析了WDR3在RNA外切酶复合体中的结构框架。研究证实WDR3的N端结构域对复合体组装及RNA底物识别至关重要,抗体在免疫印迹中用于验证突变体蛋白的表达缺失。

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**备注**:以上文献信息为示例性概括,实际引用时需根据具体研究通过PubMed或Google Scholar检索真实文献(关键词如“WDR3 antibody”、“WDR3 function”)。若需实验抗体说明书,可参考供应商(如Abcam、CST)提供的产品引用文献。

背景信息

The WDR3 (N-term) antibody is designed to target the N-terminal region of the WD repeat domain 3 protein, a member of the evolutionarily conserved WD-repeat protein family. WDR3 contains multiple WD40 repeats, structural motifs that facilitate protein-protein interactions and contribute to the assembly of multiprotein complexes. Functionally, WDR3 plays a critical role in ribosome biogenesis, particularly in the maturation of 18S rRNA, a process essential for proper ribosomal subunit assembly and cellular translation. It is a core component of the U3 small nucleolar ribonucleoprotein (snoRNP) complex, which guides site-specific modifications during rRNA processing. Dysregulation of WDR3 has been implicated in developmental disorders and diseases linked to ribosomal dysfunction, such as certain cancers and ribosomopathies. The N-terminal-specific antibody enables researchers to study WDR3's expression, localization, and interactions in various biological contexts, including cell cycle regulation, proliferation, and stress responses. It is commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate WDR3's molecular mechanisms and its association with pathological conditions. The antibody's specificity for the N-terminal region ensures targeted detection, avoiding cross-reactivity with other WD-repeat family members.

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