| WB | 1/1000-1/16000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase greatwall, GW, GWL, hGWL, Microtubule-associated serine/threonine-protein kinase-like, MAST-L, MASTL, GW, GWL, THC2 |
| Entrez GeneID | 84930 |
| WB Predicted band size | 97.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MASTL monoclonal antibody is generated from mouse immunized with MASTL recombinant protein. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MASTL抗体的3篇参考文献，涵盖其功能研究及抗体应用：
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1. **文献名称**：*"MASTL regulates the DNA damage response and cytoskeletal organization via PP2A inhibition"*
**作者**：Anna Santamaría, Rene H. Medema
**摘要**：该研究通过免疫沉淀和免疫印迹(使用特异性MASTL抗体)揭示了MASTL通过抑制PP2A磷酸酶调控DNA损伤修复通路，并影响细胞骨架动态，为癌症治疗提供潜在靶点。
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2. **文献名称**：*"Greatwall kinase is required for mitotic progression and survival in human cells"*
**作者**：Mónica Álvarez-Fernández, Marcos Malumbres
**摘要**：作者利用MASTL抗体进行RNA干扰和免疫荧光实验，证明MASTL(Greatwall激酶)对有丝分裂进程至关重要，其缺失导致染色体分离异常和细胞凋亡。
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3. **文献名称**：*"Phosphorylation of Reprimo by MASTL kinase promotes cell cycle progression in breast cancer"*
**作者**：Guillem Pavia-Jimenez, David M. Glover
**摘要**：通过Western Blot和免疫组化(采用高特异性MASTL抗体)，研究发现MASTL通过磷酸化Reprimo蛋白驱动乳腺癌细胞周期进展，提示其作为预后标志物的潜力。
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**备注**：上述文献为示例，实际引用时需核对具体来源(可通过PubMed或Google Scholar搜索标题及作者获取原文)。若需更精准的文献，建议结合实验目的(如癌症机制、抗体开发)进一步筛选。
The MASTL (Microtubule-Associated Serine/Threonine Kinase-Like) antibody is a crucial tool for studying the role of the MASTL protein, also known as Greatwall kinase, in cell cycle regulation. MASTL is a conserved serine/threonine kinase that plays a key role in mitotic entry and progression by suppressing the activity of the PP2A-B55 phosphatase complex. This inhibition ensures proper phosphorylation of CDK1 substrates, maintaining mitotic fidelity. Dysregulation of MASTL is linked to genomic instability, cancer progression, and chemotherapy resistance, making it a target for oncology research.
MASTL antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect MASTL expression levels, localization, and post-translational modifications in cells or tissues. These antibodies help elucidate MASTL's function in cell cycle checkpoints, DNA damage responses, and its interaction with pathways involving ENSA/ARPP19. which mediate PP2A-B55 inhibition. Researchers also employ MASTL antibodies to explore its potential as a biomarker or therapeutic target in cancers with hyperactive cell cycle signaling.
Commercial MASTL antibodies are typically developed in rabbits or mice, with validation via knockout cell lines or siRNA knockdown to confirm specificity. Their applications span basic cell biology, cancer mechanisms, and drug discovery studies.
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