| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-directed RNA polymerase II subunit RPB7, RNA polymerase II subunit B7, DNA-directed RNA polymerase II subunit G, RNA polymerase II 19 kDa subunit, RPB19, POLR2G, RPB7 |
| Entrez GeneID | 5436 |
| WB Predicted band size | 19.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This POLR2G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-153 amino acids from the C-terminal region of human POLR2G. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于POLR2G抗体的参考文献摘要：
1. **文献名称**：*Structural insights into RNA polymerase II assembly using site-specific crosslinking and antibody-based analysis*
**作者**：Cramer P. et al.
**摘要**：通过结合位点特异性交联和POLR2G抗体免疫沉淀技术，揭示了RNA聚合酶II亚基RPB7(POLR2G编码)在酶复合体组装中的关键作用，证明其参与启动子识别和转录起始调控。
2. **文献名称**：*A monoclonal antibody targeting POLR2G distinguishes malignant from normal cells in colorectal cancer*
**作者**：Liu Y. et al.
**摘要**：研究开发了一种特异性识别POLR2G的单克隆抗体，发现其在结直肠癌组织中高表达，且通过抑制POLR2G可降低肿瘤细胞增殖，提示其作为癌症治疗靶点的潜力。
3. **文献名称**：*The role of RNA polymerase II subunit RPB7 (POLR2G) in viral transcription*
**作者**：Martinez-Rucobo F.W. et al.
**摘要**：利用POLR2G抗体进行染色质免疫共沉淀(ChIP)，发现该亚基与多种病毒基因组的转录激活相关，尤其在RNA病毒复制中可能通过调控宿主聚合酶活性影响病毒生命周期。
(注：上述文献为虚拟示例，实际研究需通过学术数据库检索。)
The POLR2G antibody targets the POLR2G protein, a subunit of RNA polymerase II (Pol II), the enzyme responsible for synthesizing messenger RNA in eukaryotes. POLR2G (Polymerase RNA Subunit 2G), also known as RPB7. is a conserved core component of Pol II and plays a critical role in transcription initiation and elongation. It interacts with other subunits, such as POLR2A and POLR2F, to stabilize the polymerase complex and facilitate promoter-proximal pausing, a regulatory checkpoint in transcription. POLR2G also participates in coordinating transcription with RNA processing and chromatin remodeling.
Antibodies against POLR2G are widely used to study Pol II assembly, transcription dynamics, and gene regulation. They enable detection of POLR2G expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, immunofluorescence, and ChIP-seq. These tools are essential for investigating transcriptional dysregulation in diseases, including cancer, neurodegenerative disorders, and viral infections. For example, altered POLR2G expression has been linked to tumor progression and impaired neuronal differentiation.
POLR2G antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown. Both monoclonal and polyclonal variants are available, often raised in rabbits or mice. Their applications span basic research on transcriptional mechanisms to clinical studies exploring biomarkers or therapeutic targets. Proper controls, such as isotype-matched antibodies and antigen-blocking experiments, are recommended to confirm signal specificity.
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