WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/100-1/500 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Protocadherin beta-12, PCDH-beta-12, PCDHB12 |
Entrez GeneID | 56124 |
WB Predicted band size | 86.8kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This PCDHB12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 766-795 amino acids from the C-terminal region of human PCDHB12. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于PCDHB12抗体的模拟参考文献(基于领域知识构造,建议通过PubMed/Google Scholar验证准确性):
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1. **文献名称**: *PCDHB12 as a candidate tumor suppressor in glioblastoma*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过免疫组化发现PCDHB12蛋白在胶质母细胞瘤组织中表达显著下调,其低表达与患者不良预后相关。利用特异性PCDHB12抗体验证了其在抑制肿瘤细胞迁移中的作用,提示其可能通过Wnt信号通路调控癌症进展。
2. **文献名称**: *Protocadherin β12 regulates synaptic plasticity in hippocampal neurons*
**作者**: Smith JL, et al.
**摘要**: 通过在小鼠海马神经元中使用PCDHB12抗体进行免疫荧光定位,发现该蛋白富集于突触后膜。基因敲除实验表明,PCDHB12缺失导致突触可塑性受损,提示其在神经回路形成中起关键作用。
3. **文献名称**: *Development and validation of a novel PCDHB12 monoclonal antibody for clinical diagnostics*
**作者**: Chen R, et al.
**摘要**: 研究团队开发了一种高特异性抗人PCDHB12单克隆抗体,并通过ELISA、Western blot和免疫组化验证其灵敏度及特异性。该抗体成功应用于乳腺癌组织芯片分析,显示PCDHB12表达与ER阳性亚型显著相关。
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**建议检索关键词**:
- PubMed中搜索 "PCDHB12 antibody" + "expression" / "cancer" / "neuron"
- Google Scholar筛选近5年文献,限定关键词:Protocadherin beta 12. antibody validation, functional study
**注意**: 以上文献为示例性质,实际研究可能需要结合具体数据库检索。
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