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Rabbit Polyclonal PCDHB12 Antibody

  • 中文名: PCDHB12抗体
  • 别    名: Protocadherin beta-12, PCDH-beta-12, PCDHB12
货号: IPDX31070
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesProtocadherin beta-12, PCDH-beta-12, PCDHB12
Entrez GeneID56124
WB Predicted band size86.8kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis PCDHB12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 766-795 amino acids from the C-terminal region of human PCDHB12.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是3篇关于PCDHB12抗体的模拟参考文献(基于领域知识构造,建议通过PubMed/Google Scholar验证准确性):

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1. **文献名称**: *PCDHB12 as a candidate tumor suppressor in glioblastoma*

**作者**: Zhang Y, et al.

**摘要**: 本研究通过免疫组化发现PCDHB12蛋白在胶质母细胞瘤组织中表达显著下调,其低表达与患者不良预后相关。利用特异性PCDHB12抗体验证了其在抑制肿瘤细胞迁移中的作用,提示其可能通过Wnt信号通路调控癌症进展。

2. **文献名称**: *Protocadherin β12 regulates synaptic plasticity in hippocampal neurons*

**作者**: Smith JL, et al.

**摘要**: 通过在小鼠海马神经元中使用PCDHB12抗体进行免疫荧光定位,发现该蛋白富集于突触后膜。基因敲除实验表明,PCDHB12缺失导致突触可塑性受损,提示其在神经回路形成中起关键作用。

3. **文献名称**: *Development and validation of a novel PCDHB12 monoclonal antibody for clinical diagnostics*

**作者**: Chen R, et al.

**摘要**: 研究团队开发了一种高特异性抗人PCDHB12单克隆抗体,并通过ELISA、Western blot和免疫组化验证其灵敏度及特异性。该抗体成功应用于乳腺癌组织芯片分析,显示PCDHB12表达与ER阳性亚型显著相关。

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**建议检索关键词**:

- PubMed中搜索 "PCDHB12 antibody" + "expression" / "cancer" / "neuron"

- Google Scholar筛选近5年文献,限定关键词:Protocadherin beta 12. antibody validation, functional study

**注意**: 以上文献为示例性质,实际研究可能需要结合具体数据库检索。

背景信息

The PCDHB12 antibody is a research tool targeting Protocadherin Beta-12. a member of the protocadherin β gene cluster within the cadherin superfamily. Protocadherins are transmembrane proteins involved in cell-cell adhesion, neural circuit assembly, and synaptic specificity, with PCDHB12 predominantly expressed in the nervous system. This antibody is commonly used to study PCDHB12's role in neuronal development, axon guidance, and synaptic plasticity through techniques like immunohistochemistry, Western blotting, or immunofluorescence. Emerging evidence suggests PCDHB12 may contribute to neurodevelopmental disorders and cancer progression, particularly in malignancies with neural or epithelial origins. As a calcium-dependent cell adhesion molecule, PCDHB12 is hypothesized to influence tissue architecture and intracellular signaling pathways. However, its precise biological functions remain under investigation, partly due to functional redundancy within the protocadherin family. Commercial PCDHB12 antibodies are typically validated for specificity in human or mouse models, though cross-reactivity across species varies. Researchers utilize this reagent to map protein localization, quantify expression changes in disease models, or explore interactions with other cadherin-related proteins. Optimal experimental results require validation of antibody performance in specific sample types due to potential post-translational modifications or epitope masking.

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