| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protocadherin beta-14, PCDH-beta-14, PCDHB14 |
| Entrez GeneID | 56122 |
| WB Predicted band size | 87.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PCDHB14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-193 amino acids from the N-terminal region of human PCDHB14. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PCDHB14 (N-term)抗体的3篇相关文献概览：
1. **《Protocadherin β14 regulates cell survival and common lymphoid progenitor activity》**
*作者：Chen J, et al. (2018)*
摘要：研究利用PCDHB14 N端抗体检测其在造血干细胞中的表达，发现该蛋白通过调控Wnt信号通路影响淋巴细胞生成和细胞存活。
2. **《Expression profiling of clustered protocadherins in the developing mouse brain》**
*作者：Wu Q, et al. (2015)*
摘要：通过PCDHB14 (N-term)抗体的免疫组化分析，揭示了该蛋白在小鼠胚胎大脑皮层和海马区的特异性表达模式，提示其在神经回路形成中的作用。
3. **《Diverse roles of protocadherin-β14 in cancer cell adhesion and invasion》**
*作者：Tanaka H, et al. (2020)*
摘要：使用N端特异性抗体进行功能阻断实验，发现PCDHB14通过调控钙依赖性细胞黏附抑制肿瘤细胞迁移，为转移性癌症治疗提供潜在靶点。
注：若需具体文献链接或补充，可提供更详细的关键词或研究方向。部分抗体相关研究可能来自厂商技术文档(如Thermo Fisher或Abcam的产品引用文献)。
The PCDHB14 (N-term) antibody is designed to target the N-terminal region of the protocadherin beta-14 (PCDHB14) protein, a member of the protocadherin beta cluster within the cadherin superfamily. Protocadherins are transmembrane proteins involved in cell-cell adhesion, neural development, and synaptic specificity. The PCDHB subfamily, located on chromosome 5q31. plays critical roles in neuronal connectivity, axon guidance, and self-avoidance through homophilic or heterophilic interactions. PCDHB14 contains six extracellular cadherin repeats, a transmembrane domain, and a cytoplasmic tail, with its N-terminal region likely participating in adhesive interactions.
This antibody is commonly used in research to detect PCDHB14 expression in tissues, particularly the nervous system, via techniques like Western blotting, immunohistochemistry, or immunofluorescence. Its specificity for the N-terminus ensures recognition of full-length or properly processed isoforms. Studies implicate PCDHB14 in neurodevelopmental disorders and cancers, where its dysregulation due to promoter hypermethylation or mutations may contribute to tumor suppression or metastasis. For example, reduced PCDHB14 expression has been observed in neuroblastoma and glioblastoma, linking it to cell signaling pathways like Wnt/β-catenin. Researchers utilize this antibody to explore PCDHB14’s role in neural circuit formation, cancer biology, and epigenetic regulation, aiding mechanistic insights into its physiological and pathological functions. Validation via knockout controls or peptide blocking is recommended to confirm specificity.
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