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Rabbit Polyclonal SFRS7(N-term) Antibody

  • 中文名: SFRS7 (N-term)抗体
  • 别    名: Serine/arginine-rich splicing factor 7, Splicing factor 9G8, Splicing factor, arginine/serine-rich 7, SRSF7, SFRS7
货号: IPDX31067
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesSerine/arginine-rich splicing factor 7, Splicing factor 9G8, Splicing factor, arginine/serine-rich 7, SRSF7, SFRS7
Entrez GeneID6432
WB Predicted band size27.4kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis SFRS7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the N-terminal region of human SFRS7.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于SFRS7(N-term)抗体的3篇参考文献的简要概括(注:文献为模拟示例,实际引用请核实原始论文):

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1. **文献名称**:*"Characterization of SFRS7/N-term antibody in spliceosome assembly"*

**作者**:Zhang et al.

**摘要**:本研究利用SFRS7(N-term)特异性抗体,通过免疫沉淀和质谱分析,揭示了SFRS7在pre-mRNA剪接复合体组装中的作用,并发现其N端结构域对结合U1 snRNP至关重要。

2. **文献名称**:*"SFRS7 phosphorylation modulates RNA splicing in cancer cells"*

**作者**:Lee & Kim

**摘要**:通过SFRS7(N-term)抗体进行Western blot和免疫荧光实验,发现SFRS7的N端磷酸化状态在乳腺癌细胞中异常调控,影响致癌基因剪接变体的产生。

3. **文献名称**:*"Development of a polyclonal antibody targeting SFRS7 N-terminal domain"*

**作者**:Wang et al.

**摘要**:报道了一种针对SFRS7 N端抗原表位的兔源多克隆抗体的制备与验证,该抗体在检测内源性SFRS7表达及亚细胞定位中表现出高特异性和灵敏度。

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如需实际文献,建议在PubMed或Google Scholar中搜索关键词“SFRS7 antibody N-terminal”或联系抗体供应商获取引用文献。

背景信息

The SFRS7 (N-term) antibody is a tool designed to detect the N-terminal region of splicing factor arginine/serine-rich 7 (SFRS7), also known as 9G8. SFRS7 is a member of the serine/arginine (SR)-rich protein family, critical in pre-mRNA splicing. It facilitates spliceosome assembly by binding to exonic splicing enhancers (ESEs) through its RNA recognition motif (RRM), promoting inclusion or exclusion of specific exons to generate mRNA variants. This protein interacts with components of the U1 snRNP complex and other splicing regulators, influencing tissue-specific or developmental-stage-specific alternative splicing patterns. Dysregulation of SFRS7 has been implicated in cancers and neurological disorders, making it a focus in RNA metabolism and disease research. The N-term-specific antibody is commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study SFRS7 expression, localization, and interactions. Its validation typically includes detecting a ~30 kDa band (human SFRS7) and confirming specificity via knockout controls. Researchers employ this antibody to explore SFRS7's role in splicing regulation, its post-translational modifications (e.g., phosphorylation), and its pathological mechanisms in models of carcinogenesis or neurodegeneration.

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