关键信息
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基因名
RNF168
- 应用
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别名
(hRNF168)(RING finger protein 168)(RING-type E3 ubiquitin transferase RNF168)
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种属
Human
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表达系统
Baculovirus
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标签
C- His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8IYW5
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表达区间
1-571aa
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分子量
66.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RNF168, an E3 ubiquitin ligase, plays a crucial role in the DNA damage response (DDR) by facilitating the ubiquitination of proteins involved in DNA repair pathways. Its function is particularly important in signaling the presence of DNA double-strand breaks, where it helps recruit repair factors to the site of damage. Mutations or dysregulation of RNF168 have been associated with several human diseases, including immunodeficiencies and cancer, underscoring the need for detailed investigations into its biochemical properties and interactions. Recent studies have emphasized the significance of RNF168 in mediating the repair process through the modification of histones, leading to a better understanding of chromatin dynamics during the DDR. Research into the recombinant protein of RNF168 allows scientists to explore its enzymatic activity, interactome, and mechanism of action, paving the way for potential therapeutic strategies targeting its regulatory pathways. As such, RNF168 stands as a vital focus of study in the fields of molecular biology and cancer research, with ongoing efforts to elucidate its full spectrum of functions and implications in maintaining genomic stability.
