| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PRAME family member 12, PRAMEF12 |
| Entrez GeneID | 390999 |
| WB Predicted band size | 54.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PRAMEF12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-135 amino acids from the Central region of human PRAMEF12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PRAMEF12抗体的假设性参考文献示例(注：部分内容可能基于研究领域常见模式，建议通过学术数据库核实具体文献)：
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1. **标题**：*"PRAMEF12 Expression in Non-Small Cell Lung Cancer: Prognostic Implications and Immunohistochemical Analysis"*
**作者**：Smith A, et al. (2020)
**摘要**：本研究通过自制兔源多克隆PRAMEF12抗体进行免疫组化(IHC)，发现PRAMEF12在肺癌组织中显著高表达，且与患者生存率降低相关，提示其作为潜在肿瘤标志物的价值。
2. **标题**：*"Development of a Monoclonal Antibody Against PRAMEF12 for Flow Cytometry Applications"*
**作者**：Jones B, et al. (2018)
**摘要**：报道一种新型小鼠单克隆PRAMEF12抗体的开发与验证，该抗体成功用于流式细胞术检测白血病细胞表面PRAMEF12蛋白表达，为血液肿瘤研究提供工具。
3. **标题**：*"Epigenetic Regulation of PRAMEF12 in Melanoma: Insights from ChIP-seq and Antibody-Based Assays"*
**作者**：Lee C, et al. (2019)
**摘要**：利用商业PRAMEF12抗体进行染色质免疫沉淀(ChIP-seq)和Western blot，揭示PRAMEF12在黑色素瘤中受DNA甲基化调控，可能参与肿瘤侵袭性生长。
4. **标题**：*"Comprehensive Analysis of PRAMEF Family Genes in Gastrointestinal Cancers Using Custom Antibodies"*
**作者**：Brown D, et al. (2021)
**摘要**：通过定制PRAMEF12抗体对胃肠道肿瘤样本进行多组学分析，发现PRAMEF12与其他家族成员协同作用，可能成为治疗靶点。
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**建议**：以上内容为模拟示例，实际文献需通过PubMed、Web of Science等平台以关键词“PRAMEF12 antibody”或“PRAMEF12 immunological detection”检索核实。
The PRAMEF12 (PRAME Family Member 12) antibody is associated with studying proteins encoded by the PRAME (Preferentially Expressed Antigen in Melanoma) gene family, which is implicated in various cancers. PRAME family members, including PRAMEF12. are characterized by conserved leucine-rich repeats and nuclear localization signals, often functioning as cancer-testis antigens. These antigens are typically expressed in germline cells and re-expressed in malignancies, making them potential targets for immunotherapy and biomarkers for cancer diagnosis. PRAMEF12. located on chromosome 1p36. shares structural homology with other PRAME proteins but exhibits distinct expression patterns. Its role remains less defined, though it may participate in epigenetic regulation, apoptosis, or cell cycle control, similar to other PRAME members.
Antibodies targeting PRAMEF12 are primarily used in research to map its expression in normal and cancerous tissues, elucidate its molecular interactions, and assess its diagnostic or therapeutic relevance. Studies suggest PRAMEF12 overexpression in certain tumors, potentially linked to oncogenesis or immune evasion. However, challenges include cross-reactivity with other PRAME proteins and limited functional data. Current investigations focus on validating its specificity, exploring its mechanistic contributions to cancer biology, and evaluating its utility in clinical settings. The development of reliable PRAMEF12 antibodies is critical for advancing these studies and clarifying its role in health and disease.
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