| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 ubiquitin-protein ligase TRIM69, 632-, RFP-like domain-containing protein trimless, RING finger protein 36, Tripartite motif-containing protein 69, TRIM69, RNF36 |
| Entrez GeneID | 140691 |
| WB Predicted band size | 57.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This TRIM69 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-129 amino acids from the N-terminal region of human TRIM69. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于TRIM69 (N-term)抗体的3篇参考文献示例(注：部分文献信息为模拟概括，实际引用请以真实文献为准)：
---
1. **"TRIM69 inhibits Zika virus replication through ubiquitination of viral NS3 protein"**
*Zhang Y, et al. (2021) Cell Reports*
摘要：本研究利用TRIM69 (N-term)抗体进行免疫共沉淀和Western blot分析，发现TRIM69通过靶向寨卡病毒NS3蛋白的泛素化降解抑制病毒复制，揭示了其在抗病毒天然免疫中的作用。
2. **"TRIM69 promotes apoptosis in hepatocellular carcinoma by modulating p53 stability"**
*Wang L, et al. (2020) Oncogene*
摘要：通过TRIM69 (N-term)抗体的免疫组化及敲除实验，证实TRIM69在肝癌组织中低表达，并通过与p53蛋白相互作用增强其稳定性，从而诱导肿瘤细胞凋亡。
3. **"Structural and functional characterization of TRIM69 in spermatogenesis"**
*Li X, et al. (2019) Development*
摘要：利用N端特异性抗体进行组织定位分析，发现TRIM69在小鼠睾丸中高表达，并参与精子形成过程中的染色质重塑，基因敲除模型显示其缺陷导致雄性不育。
---
**注**：以上文献信息为示例性质，实际研究中需通过PubMed或Google Scholar等平台检索真实发表的论文(可尝试关键词：TRIM69 antibody、TRIM69 N-terminal function)。若需商业抗体信息，可参考供应商(如Santa Cruz Biotechnology、Abcam)的产品说明书引用文献。
The TRIM69 (N-term) antibody is designed to target the N-terminal region of the TRIM69 protein, a member of the tripartite motif (TRIM) family. TRIM proteins are characterized by conserved RING, B-box, and coiled-coil domains, which are involved in diverse cellular processes, including ubiquitination, innate immunity, and apoptosis. TRIM69. also known as RNF36. has been implicated in regulating apoptosis, viral restriction, and immune responses. The N-terminal region of TRIM69 contains the RING domain, which confers E3 ubiquitin ligase activity, enabling post-translational modification of target proteins.
This antibody is commonly used in research to detect TRIM69 expression via techniques like Western blotting, immunofluorescence, or immunoprecipitation. Its specificity for the N-terminal region allows researchers to study full-length TRIM69 or distinguish it from splice variants or degradation products. TRIM69 has been studied in contexts such as antiviral defense (e.g., inhibiting Zika or dengue virus replication), testicular development, and cancer, where its dysregulation may influence tumor progression.
Validation of the antibody typically includes testing in knockout (KO) cell lines or tissues to confirm target specificity. Researchers use it to explore TRIM69's role in cellular pathways, such as modulating NF-κB signaling or interacting with viral proteins. Its application aids in understanding TRIM69's physiological and pathological functions, particularly in immune regulation and disease mechanisms.
×
