关键信息
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基因名
TAP
- 应用
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别名
TAP；ABCB2；PSF1；RING4；Antigen peptide transporter 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q03518
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表达区间
1-808aa
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氨基酸序列
MAELLASAGSACSWDFPRAPPSFPPPAASRGGLGGTRSFRPHRGAESPRP GRDRDGVRVPMASSRCPAPRGCRCLPGASLAWLGTVLLLLADWVLLRTAL PRIFSLLVPTALPLLRVWAVGLSRWAVLWLGACGVLRATVGSKSENAGAQ GWLAALKPLAAALGLALPGLALFRELISWGAPGSADSTRLLHWGSHPTAF VVSYAAALPAAALWHKLGSLWVPGGQGGSGNPVRRLLGCLGSETRRLSLF LVLVVLSSLGEMAIPFFTGRLTDWILQDGSADTFTRNLTLMSILTIASAV LEFVGDGIYNNTMGHVHSHLQGEVFGAVLRQETEFFQQNQTGNIMSRVTE DTSTLSDSLSENLSLFLWYLVRGLCLLGIMLWGSVSLTMVTLITLPLLFL LPKKVGKWYQLLEVQVRESLAKSSQVAIEALSAMPTVRSFANEEGEAQKF REKLQEIKTLNQKEAVAYAVNSWTTSISGMLLKVGILYIGGQLVTSGAVS SGNLVTFVLYQMQFTQAVEVLLSIYPRVQKAVGSSEKIFEYLDRTPRCPP SGLLTPLHLEGLVQFQDVSFAYPNRPDVLVLQGLTFTLRPGEVTALVGPN GSGKSTVAALLQNLYQPTGGQLLLDGKPLPQYEHRYLHRQVAAVGQEPQV FGRSLQENIAYGLTQKPTMEEITAAAVKSGAHSFISGLPQGYDTEVDEAG SQLSGGQRQAVALARALIRKPCVLILDDATSALDANSQLQVEQLLYESPE RYSRSVLLITQHLSLVEQADHILFLEGGAIREGGTHQQLMEKKGCYWAMV QAPADAPE
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
TAP (Tandem Affinity Purification) was developed as a powerful method for isolating and analyzing protein complexes in cellular systems. The method relies on the fusion of a target protein to a TAP tag, which contains two affinity tags, allowing for a highly selective purification process. The introduction of TAP revolutionized the study of protein interactions and complexes, providing insights into cellular mechanisms and pathways. Researchers leveraged this technique to explore the interactome, revealing the complexity of protein networks involved in crucial biological processes such as signal transduction, gene regulation, and cellular response to stimuli. TAP has been instrumental in characterizing various proteins in different organisms, thus enhancing our understanding of both basic biology and disease mechanisms. Furthermore, advancements in mass spectrometry have complemented TAP, enabling more detailed analysis of protein identities and their post-translational modifications. As a result, TAP has emerged as a quintessential tool in molecular biology and biochemistry, paving the way for new discoveries in the field of proteomics and enhancing our comprehension of life at the molecular level.
